LSBio
COG8 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

COG8 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all COG8 antibodies →
$504.00each
Catalog Number: LS-G108868-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human COG8 (NM_032382), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CDG2H | COG8 | Dor1 | COG complex subunit 8 | Dependent on RIC1
Origin Species
Human
NCBI GenBank
NM_032382
SwissProt
Q96MW5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
68.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MATAATIPSV ATATAAALGE VEDEGLLASL FRDRFPEAQW RERPDVGRYL RELSGSGLER
61LRREPERLAE ERAQLLQQTR DLAFANYKTF IRGAECTERI HRLFGDVEAS LGRLLDRLPS
121FQQSCRNFVK EAEEISSNRR MNSLTLNRHT EILEILEIPQ LMDTCVRNSY YEEALELAAY
181VRRLERKYSS IPVIQGIVNE VRQSMQLMLS QLIQQLRTNI QLPACLRVIG YLRRMDVFTE
241AELRVKFLQA RDAWLRSILT AIPNDDPYFH ITKTIEASRV HLFDIITQYR AIFSDEDPLL
301PPAMGEHTVN ESAIFHGWVL QKVSQFLQVL ETDLYRGIGG HLDSLLGQCM YFGLSFSRVG
361ADFRGQLAPV FQRVAISTFQ KAIQETVEKF QEEMNSYMLI SAPAILGTSN MPAAVPATQP
421GTLQPPMVLL DFPPLACFLN NILVAFNDLR LCCPVALAQD VTGALEDALA KVTKIILAFH
481RAEEAAFSSG EQELFVQFCT VFLEDLVPYL NRCLQVLFPP AQIAQTLGIP PTQLSKYGNL
541GHVNIGAIQE PLAFILPKRE TLFTLDDQAL GPELTAPAPE PPAEEPRLEP AGPACPEGGR
601AETQAEPPSV GP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

612 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.