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COG7 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

COG7 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all COG7 antibodies →
$504.00each
Catalog Number: LS-G105747-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human COG7 (NM_153603), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
COG complex subunit 7 | CDG2E | COG7
Origin Species
Human
SwissProt
P83436

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
86.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDFSKFLADD FDVKEWINAA FRAGSKEAAS GKADGHAATL VMKLQLFIQE VNHAVEETSH
61QALQNMPKVL RDVEALKQEA SFLKEQMILV KEDIKKFEQD TSQSMQVLVE IDQVKSRMQL
121AAESLQEADK WSTLSADIEE TFKTQDIAVI SAKLTGMQNS LMMLVDTPDY SEKCVHLEAL
181KNRLEALASP QIVAAFTSQA VDQSKVFVKV FTEIDRMPQL LAYYYKCHKV QLLAAWQELC
241QSDLSLDRQL TGLYDALLGA WHTQIQWATQ VFQKPHEVVM VLLIQTLGAL MPSLPSCLSN
301GVERAGPEQE LTRLLEFYDA TAHFAKGLEM ALLPHLHEHN LVKVTELVDA VYDPYKPYQL
361KYGDMEESNL LIQMSAVPLE HGEVIDCVQE LSHSVNKLFG LASAAVDRCV RFTNGLGTCG
421LLSALKSLFA KYVSDFTSTL QSIRKKCKLD HIPPNSLFQE DWTAFQNSIR IIATCGELLR
481HCGDFEQQLA NRILSTAGKY LSDSCSPRSL AGFQESILTD KKNSAKNPWQ EYNYLQKDNP
541AEYASLMEIL YTLKEKGSSN HNLLAAPRAA LTRLNQQAHQ LAFDSVFLRI KQQLLLISKM
601DSWNTAGIGE TLTDELPAFS LTPLEYISNI GQYIMSLPLN LEPFVTQEDS ALELALHAGK
661LPFPPEQGDE LPELDNMADN WLGSIARATM QTYCDAILQI PELSPHSAKQ LATDIDYLIN
721VMDALGLQPS RTLQHIVTLL KTRPEDYRQV SKGLPRRLAT TVATMRSVNY
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

770 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.