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COG3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

COG3 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G109201-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human COG3 (NM_031431), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
COG complex subunit 3 | COG3 | SEC34 | Tethering factor SEC34 | p94
Origin Species
Human
NCBI GenBank
NM_031431
SwissProt
Q96JB2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
93.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAEAALLLLP EAAAERDARE KLALWDRRPD TTAPLTDRQT DSVLELKAAA ENLPVPAELP
61IEDLCSLTSQ SLPIELTSVV PESTEDILLK GFTSLGMEEE RIETAQQFFS WFAKLQTQMD
121QDEGTKYRQM RDYLSGFQEQ CDAILNDVNS ALQHLESLQK QYLFVSNKTG TLHEACEQLL
181KEQSELVDLA ENIQQKLSYF NELETINTKL NSPTLSVNSD GFIPMLAKLD DCITYISSHP
241NFKDYPIYLL KFKQCLSKAL HLMKTYTVNT LQTLTSQLLK RDPSSVPNAD NAFTLFYVKF
301RAAAPKVRTL IEQIELRSEK IPEYQQLLND IHQCYLDQRE LLLGPSIACT VAELTSQNNR
361DHCALVRSGC AFMVHVCQDE HQLYNEFFTK PTSKLDELLE KLCVSLYDVF RPLIIHVIHL
421ETLSELCGIL KNEVLEDHVQ NNAEQLGAFA AGVKQMLEDV QERLVYRTHI YIQTDITGYK
481PAPGDLAYPD KLVMMEQIAQ SLKDEQKKVP SEASFSDVHL EEGESNSLTK SGSTESLNPR
541PQTTISPADL HGMWYPTVRR TLVCLSKLYR CIDRAVFQGL SQEALSACIQ SLLGASESIS
601KNKTQIDGQL FLIKHLLILR EQIAPFHTEF TIKEISLDLK KTRDAAFKIL NPMTVPRFFR
661LNSNNALIEF LLEGTPEIRE HYLDSKKDVD RHLKSACEQF IQQQTKLFVE QLEEFMTKVS
721ALKTMASQGG PKYTLSQQPW AQPAKVNDLA ATAYKTIKTK LPVTLRSMSL YLSNKDTEFI
781LFKPVRNNIQ QVFQKFHALL KEEFSPEDIQ IIACPSMEQL SLLLSVSK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

828 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.