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COG2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

COG2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all COG2 antibodies →
$504.00each
Catalog Number: LS-G101020-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human COG2 (NM_007357), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
COG complex subunit 2 | COG2 | LDLC
Origin Species
Human
NCBI GenBank
NM_007357
SwissProt
Q14746

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
83 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEKSRMNLPK GPDTLCFDKD EFMKEDFDVD HFVSDCRKRV QLEELRDDLE LYYKLLKTAM
61VELINKDYAD FVNLSTNLVG MDKALNQLSV PLGQLREEVL SLRSSVSEGI RAVDERMSKQ
121EDIRKKKMCV LRLIQVIRSV EKIEKILNSQ SSKETSALEA SSPLLTGQIL ERIATEFNQL
181QFHAVQSKGM PLLDKVRPRI AGITAMLQQS LEGLLLEGLQ TSDVDIIRHC LRTYATIDKT
241RDAEALVGQV LVKPYIDEVI IEQFVESHPN GLQVMYNKLL EFVPHHCRLL REVTGGAISS
301EKGNTVPGYD FLVNSVWPQI VQGLEEKLPS LFNPGNPDAF HEKYTISMDF VRRLERQCGS
361QASVKRLRAH PAYHSFNKKW NLPVYFQIRF REIAGSLEAA LTDVLEDAPA ESPYCLLASH
421RTWSSLRRCW SDEMFLPLLV HRLWRLTLQI LARYSVFVNE LSLRPISNES PKEIKKPLVT
481GSKEPSITQG NTEDQGSGPS ETKPVVSISR TQLVYVVADL DKLQEQLPEL LEIIKPKLEM
541IGFKNFSSIS AALEDSQSSF SACVPSLSSK IIQDLSDSCF GFLKSALEVP RLYRRTNKEV
601PTTASSYVDS ALKPLFQLQS GHKDKLKQAI IQQWLEGTLS ESTHKYYETV SDVLNSVKKM
661EESLKRLKQA RKTTPANPVG PSGGMSDDDK IRLQLALDVE YLGEQIQKLG LQASDIKSFS
721ALAELVAAAK DQATAEQP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

738 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.