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COG1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

COG1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all COG1 antibodies →
$504.00each
Catalog Number: LS-G111567-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human COG1 (NM_018714), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CDG2G | COG1 | COG complex subunit 1 | KIAA1381 | LDLB
Origin Species
Human
SwissProt
Q8WTW3

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
108.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MATAATSPAL KRLDLRDPAA LFETHGAEEI RGLERQVRAE IEHKKEELRQ MVGERYRDLI
61EAADTIGQMR RCAVGLVDAV KATDQYCARL RQAGSAAPRP PRAQQPQQPS QEKFYSMAAQ
121IKLLLEIPEK IWSSMEASQC LHATQLYLLC CHLHSLLQLD SSSSRYSPVL SRFPILIRQV
181AAASHFRSTI LHESKMLLKC QGVSDQAVAE ALCSIMLLEE SSPRQALTDF LLARKATIQK
241LLNQPHHGAG IKAQICSLVE LLATTLKQAH ALFYTLPEGL LPDPALPCGL LFSTLETITG
301QHPAGKGTGV LQEEMKLCSW FKHLPASIVE FQPTLRTLAH PISQEYLKDT LQKWIHMCNE
361DIKNGITNLL MYVKSMKGLA GIRDAMWELL TNESTNHSWD VLCRRLLEKP LLFWEDMMQQ
421LFLDRLQTLT KEGFDSISSS SKELLVSALQ ELESSTSNSP SNKHIHFEYN MSLFLWSESP
481NDLPSDAAWV SVANRGQFAS SGLSMKAQAI SPCVQNFCSA LDSKLKVKLD DLLAYLPSDD
541SSLPKDVSPT QAKSSAFDRY ADAGTVQEML RTQSVACIKH IVDCIRAELQ SIEEGVQGQQ
601DALNSAKLHS VLFMARLCQS LGELCPHLKQ CILGKSESSE KPAREFRALR KQGKVKTQEI
661IPTQAKWQEV KEVLLQQSVM GYQVWSSAVV KVLIHGFTQS LLLDDAGSVL ATATSWDELE
721IQEEAESGSS VTSKIRLPAQ PSWYVQSFLF SLCQEINRVG GHALPKVTLQ EMLKSCMVQV
781VAAYEKLSEE KQIKKEGAFP VTQNRALQLL YDLRYLNIVL TAKGDEVKSG RSKPDSRIEK
841VTDHLEALID PFDLDVFTPH LNSNLHRLVQ RTSVLFGLVT GTENQLAPRS STFNSQEPHN
901ILPLASSQIR FGLLPLSMTS TRKAKSTRNI ETKAQVVPPA RSTAGDPTVP GSLFRQLVSE
961EDNTSAPSLF KLGWLSSMTK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

980 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.