LSBio
CNTROB Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CNTROB Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CNTROB antibodies →
$504.00each
Catalog Number: LS-G102169-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CNTROB (NM_053051), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Centrobin | LYST-interacting protein 8 | LYST-interacting protein LIP8 | LIP8 | CNTROB | PP1221
Origin Species
Human
SwissProt
Q8N137

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
101.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MATSADSPSS PLGAEDLLSD SSEPPGLNQV SSEVTSQLYA SLRLSRQAEA TARAQLYLPS
61TSPPHEGLDG FAQELSRSLS VGLEKNLKKK DGSKHIFEME SVRGQLQTML QTSRDTAYRD
121PLIPGAGSER REEDSFDSDS TATLLNTRPL QDLSPSSSAQ ALEELFPRYT SLRPGPPLNP
181PDFQGLRDAL DSEHTRRKHC ERHIQSLQTR VLELQQQLAV AVAADRKKDT MIEQLDKTLA
241RVVEGWNRHE AERTEVLRGL QEEHQAAELT RSKQQETVTR LEQSLSEAME ALNREQESAR
301LQQRERETLE EERQALTLRL EAEQQRCCVL QEERDAARAG QLSEHRELET LRAALEEERQ
361TWAQQEHQLK EHYQALQEES QAQLEREKEK SQREAQAAWE TQHQLALVQS EVRRLEGELD
421TARRERDALQ LEMSLVQARY ESQRIQLESE LAVQLEQRVT ERLAQAQESS LRQAASLREH
481HRKQLQDLSG QHQQELASQL AQFKVEMAER EERQQQVAED YELRLAREQA RVCELQSGNQ
541QLEEQRVELV ERLQAMLQAH WDEANQLLST TLPPPNPPAP PAGPSSPGPQ EPEKEERRVW
601TMPPMAVALK PVLQQSREAR DELPGAPPVL CSSSSDLSLL LGPSFQSQHS FQPLEPKPDL
661TSSTAGAFSA LGAFHPDHRA ERPFPEEDPG PDGEGLLKQG LPPAQLEGLK NFLHQLLETV
721PQNNENPSVD LLPPKSGPLT VPSWEEAPQV PRIPPPVHKT KVPLAMASSL FRVPEPPSSH
781SQGSGPSSGS PERGGDGLTF PRQLMEVSQL LRLYQARGWG ALPAEDLLLY LKRLEHSGTD
841GRGDNVPRRN TDSRLGEIPR KEIPSQAVPR RLATAPKTEK PPARKKSGHP APSSMRSRGG
901VWR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

903 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.