Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CNTN6 / Contactin 6 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G113930-100
Catalog Number: LS-G113930
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CNTN6 (NM_014461), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Contactin 6 | CNTN6 | NB3 | HNB-3 | Neural adhesion molecule | Contactin-6 | NB-3
- Origin Species
- Human
- NCBI GenBank
- NM_014461
- SwissProt
- Q9UQ52
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 113.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MRLLWKLVIL LPLINSSAGD GLLSRPIFTQ EPHDVIFPLD LSKSEVILNC AANGYPSPHY61RWKQNGTDID FTMSYHYRLD GGSLAINSPH TDQDIGMYQC LATNLLGTIL SRKAKLQFAY121IEDFETKTRS TVSVREGQGV VLLCGPPPHF GDLSYAWTFN DNPLYVQEDN RRFVSQETGN181LYIAKVEPSD VGNYTCFITN KEAQRSVQGP PTPLVQRTDG VMGEYEPKIE VRFPETIQAA241KDSSVKLECF ALGNPVPDIS WRRLDGSPLP GKVKYSKSQA ILEIPNFQQE DEGFYECIAS301NLRGRNLAKG QLIFYAPPEW EQKIQNTHLS IYDNLLWECK ASGKPNPWYT WLKNGERLNP361EERIQIENGT LIITMLNVSD SGVYQCAAEN KYQIIYANAE LRVLASAPDF SKSPVKKKSF421VQVGGDIVIG CKPNAFPRAA ISWKRGTETL RQSKRIFLLE DGSLKIYNIT RSDAGSYTCI481ATNQFGTAKN TGSLIVKERT VITVPPSKMD VTVGESIVLP CQVSHDPSIE VVFVWFFNGD541VIDLKKGVAH FERIGGESVG DLMIRNIQLH HSGKYLCTVQ TTLESLSAVA DIIVRGPPGP601PEDVQVEDIS STTSQLSWRA GPDNNSPIQI FTIQTRTPFS VGWQAVATVP EILNGKTYNA661TVVGLSPWVE YEFRVVAGNS IGIGEPSEPS ELLRTKASVP VVAPVNIHGG GGSRSELVIT721WESIPEELQN GEGFGYIIMF RPVGSTTWSK EKVSSVESSR FVYRNESIIP LSPFEVKVGV781YNNEGEGSLS TVTIVYSGED EPQLAPRGTS LQSFSASEME VSWNAIAWNR NTGRVLGYEV841LYWTDDSKES MIGKIRVSGN VTTKNITGLK ANTIYFASVR AYNTAGTGPS SPPVNVTTKK901SPPSQPPANI AWKLTNSKLC LNWEHVKTME NESEVLGYKI LYRQNRQSKT HILETNNTSA961ELLVPFEEDY LIEIRTVSDG GDGSSSEEIR IPKMSSLSSR GIQFLEPSTH FLSIVIVIFH1021CFAIQPLITRTRPLEQKLISEEDLAANDILDYKDDDDKV
1028 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.