Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CNTN1 / gp135 / Contactin 1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G105112-100
Catalog Number: LS-G105112
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Contactin 1(CNTN1) (NM_175038), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CNTN1 | Contactin | Contactin 1 | Glycoprotein gp135 | gp135 | Neural cell surface protein F3 | Contactin-1
- Origin Species
- Human
- NCBI GenBank
- NM_001843
- SwissProt
- Q12860
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 109.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MKMWLLVSHL VIISITTCLA VSEEDKGFGP IFEEQPINTI YPEESLEGKV SLNCRARASP61FPVYKWRMNN GDVDLTSDRY SMVGGNLVIN NPDKQKDAGI YYCLASNNYG MVRSTEATLS121FGYLDPFPPE ERPEVRVKEG KGMVLLCDPP YHFPDDLSYR WLLNEFPVFI TMDKRRFVSQ181TNGNLYIANV EASDKGNYSC FVSSPSITKS VFSKFIPLIP IPERTTKPYP ADIVVQFKDV241YALMGQNVTL ECFALGNPVP DIRWRKVLEP MPSTAEISTS GAVLKIFNIQ LEDEGIYECE301AENIRGKDKH QARIYVQAFP EWVEHINDTE VDIGSDLYWP CVATGKPIPT IRWLKNGYAY361HKGELRLYDV TFENAGMYQC IAENTYGAIY ANAELKILAL APTFEMNPMK KKILAAKGGR421VIIECKPKAA PKPKFSWSKG TEWLVNSSRI LIWEDGSLEI NNITRNDGGI YTCFAENNRG481KANSTGTLVI TDPTRIILAP INADITVGEN ATMQCAASFD PALDLTFVWS FNGYVIDFNK541ENIHYQRNFM LDSNGELLIR NAQLKHAGRY TCTAQTIVDN SSASADLVVR GPPGPPGGLR601IEDIRATSVA LTWSRGSDNH SPISKYTIQT KTILSDDWKD AKTDPPIIEG NMEAARAVDL661IPWMEYEFRV VATNTLGRGE PSIPSNRIKT DGAAPNVAPS DVGGGGGRNR ELTITWAPLS721REYHYGNNFG YIVAFKPFDG EEWKKVTVTN PDTGRYVHKD ETMSPSTAFQ VKVKAFNNKG781DGPYSLVAVI NSAQDAPSEA PTEVGVKVLS SSEISVHWEH VLEKIVESYQ IRYWAAHDKE841EAANRVQVTS QEYSARLENL LPDTQYFIEV GACNSAGCGP PSDMIEAFTK KAPPSQPPRI901ISSVRSGSRY IITWDHVVAL SNESTVTGYK VLYRPDGQHD GKLYSTHKHS IEVPIPRDGE961YVVEVRAHSD GGDGVVSQVK ISGAPTLSPS LLGLLLPAFG ILVYLEFTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1007 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.