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CNOT6 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CNOT6 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CNOT6 antibodies →
$725.00each
Catalog Number: LS-G113206-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CNOT6 (NM_015455), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CNOT6 | KIAA1194 | Cytoplasmic deadenylase
Origin Species
Human
NCBI GenBank
NM_015455
SwissProt
Q9ULM6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
63.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPKEKYEPPD PRRMYTIMSS EEAANGKKSH WAELEISGKV RSLSASLWSL THLTALHLSD
61NSLSRIPSDI AKLHNLVYLD LSSNKIRSLP AELGNMVSLR ELHLNNNLLR VLPFELGKLF
121QLQTLGLKGN PLTQDILNLY QEPDGTRRLL NYLLDNLSGT AKRITTEQPP PRSWIMLQEP
181DRTRPTALFS VMCYNVLCDK YATRQLYGYC PSWALNWDYR KKAIIQEILS CNADIVSLQE
241VETEQYYSFF LVELKERGYN GFFSPKSRAR TMSEQERKHV DGCAIFFKTE KFTLVQKHTV
301EFNQLAMANS EGSEAMLNRV MTKDNIGVAV LLELRKESIE MPSGKPHLGT EKQLILVANA
361HMHWDPEYSD VKLVQTMMFL SEVKNIIDKA SRNLKSSVLG EFGTIPLVLC ADLNSLPDSG
421VVEYLSTGGV ETNHKDFKEL RYNESLTNFS CHGKNGTTNG RITHGFKLQS AYESGLMPYT
481NYTFDFKGII DYIFYSKPQL NTLGILGPLD HHWLVENNIS GCPHPLIPSD HFSLFAQLEL
541LLPFLPQVNG IHLPGRR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

557 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.