Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CNOT4 / CLONE243 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G121905-100
Catalog Number: LS-G121905
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CNOT4 (NM_001008225), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CCR4-associated factor 4 | CNOT4 | NOT4 | Not4-n | NOT4H | CLONE243
- Origin Species
- Human
- NCBI GenBank
- NM_013316
- SwissProt
- O95628
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 62.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSRSPDAKED PVECPLCMEP LEIDDINFFP CTCGYQICRF CWHRIRTDEN GLCPACRKPY61PEDPAVYKPL SQEELQRIKN EKKQKQNERK QKISENRKHL ASVRVVQKNL VFVVGLSQRL121ADPEVLKRPE YFGKFGKIHK VVINNSTSYA GSQGPSASAY VTYIRSEDAL RAIQCVNNVV181VDGRTLKASL GTTKYCSYFL KNMQCPKPDC MYLHELGDEA ASFTKEEMQA GKHQEYEQKL241LQELYKLNPN FLQLSTGSVD KNKNKVTPLQ SPIDKPSDSL SIGNGDNSQQ ISNSDTPSPP301PGLSKSNPVI PISSSNHSAR SPFEGAVTES QSLFSDIFRH PNPIPSGLPP FPSSPQTSSD361WPTAPEPQSL FTSETIPVSS STDWQAAFGF GSSKQPEDDL GFDPFDVTRK ALADLIEKEL421SVQDQPSLSP TSLQNSSSHT TTAKGPGSGF LHPAAATNAN SLNSTFSVLP QRFPQFQQHR481AVYNSFSFPG QAARYPWMAF PRNSIMHLNH TANPTSNSNF LDLNLPPQHN TGLGGIPVAG541EEEVKVSTMP LSTSSHSLQQ GQQPTSLHTT VATRTRPLEQKLISEEDLAANDILDYKDDDDKV
572 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.