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CNOT3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CNOT3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CNOT3 antibodies →
$504.00each
Catalog Number: LS-G113885-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CCR4 NOT transcription complex subunit 3(CNOT3) (NM_014516), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CNOT3 | NOT3 | LENG2 | CCR4-associated factor 3 | KIAA0691 | NOT3H
Origin Species
Human
NCBI GenBank
NM_014516
SwissProt
O75175

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
81.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MADKRKLQGE IDRCLKKVSE GVEQFEDIWQ KLHNAANANQ KEKYEADLKK EIKKLQRLRD
61QIKTWVASNE IKDKRQLIDN RKLIETQMER FKVVERETKT KAYSKEGLGL AQKVDPAQKE
121KEEVGQWLTN TIDTLNMQVD QFESEVESLS VQTRKKKGDK DKQDRIEGLK RHIEKHRYHV
181RMLETILRML DNDSILVDAI RKIKDDVEYY VDSSQDPDFE ENEFLYDDLD LEDIPQALVA
241TSPPSHSHME DEIFNQSSST PTSTTSSSPI PPSPANCTTE NSEDDKKRGR STDSEVSQSP
301AKNGSKPVHS NQHPQSPAVP PTYPSGPPPA ASALSTTPGN NGVPAPAAPP SALGPKASPA
361PSHNSGTPAP YAQAVAPPAP SGPSTTQPRP PSVQPSGGGG GGSGGGGSSS SSNSSAGGGA
421GKQNGATSYS SVVADSPAEV ALSSSGGNNA SSQALGPPSG PHNPPPSTSK EPSAAAPTGA
481GGVAPGSGNN SGGPSLLVPL PVNPPSSPTP SFSDAKAAGA LLNGPPQFST APEIKAPEPL
541SSLKSMAERA AISSGIEDPV PTLHLTERDI ILSSTSAPPA SAQPPLQLSE VNIPLSLGVC
601PLGPVPLTKE QLYQQAMEEA AWHHMPHPSD SERIRQYLPR NPCPTPPYHH QMPPPHSDTV
661EFYQRLSTET LFFIFYYLEG TKAQYLAAKA LKKQSWRFHT KYMMWFQRHE EPKTITDEFE
721QGTYIYFDYE KWGQRKKEGF TFEYRYLEDR DLQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

753 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.