LSBio
CNOT10 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CNOT10 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CNOT10 antibodies →
$504.00each
Catalog Number: LS-G113212-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CNOT10 (NM_015442), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CNOT10
Origin Species
Human
NCBI GenBank
NM_015442
SwissProt
Q9H9A5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
82.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAADKPADQG AEKHEGTGQS SGITDQEKEL STNAFQAFTS GNYDACLQHL ACLQDINKDD
61YKIILNTAVA EFFKSNQTTT DNLRQTLNQL KNQVHSAVEE MDGLDDVENS MLYYNQAVIL
121YHLRQYTEAI SVGEKLYQFI EPFEEKFAQA VCFLLVDLYI LTYQAEKALH LLAVLEKMIS
181QGNNNKNGKN ETGNNNNKDG SNHKAESGAL IEAAKSKIHQ YKVRAYIQMK SLKACKREIK
241SVMNTAGNSA PSLFLKSNFE YLRGNYRKAV KLLNSSNIAE HPGFMKTGEC LRCMFWNNLG
301CIHFAMSKHN LGIFYFKKAL QENDNVCAQL SAGSTDPGKK FSGRPMCTLL TNKRYELLYN
361CGIQLLHIGR PLAAFECLIE AVQVYHANPR LWLRLAECCI AANKGTSEQE TKGLPSKKGI
421VQSIVGQGYH RKIVLASQSI QNTVYNDGQS SAIPVASMEF AAICLRNALL LLPEEQQDPK
481QENGAKNSNQ LGGNTESSES SETCSSKSHD GDKFIPAPPS SPLRKQELEN LKCSILACSA
541YVALALGDNL MALNHADKLL QQPKLSGSLK FLGHLYAAEA LISLDRISDA ITHLNPENVT
601DVSLGISSNE QDQGSDKGEN EAMESSGKRA PQCYPSSVNS ARTVMLFNLG SAYCLRSEYD
661KARKCLHQAA SMIHPKEVPP EAILLAVYLE LQNGNTQLAL QIIKRNQLLP AVKTHSEVRK
721KPVFQPVHPI QPIQMPAFTT VQRK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

744 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.