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CNKSR1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CNKSR1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CNKSR1 antibodies →
$725.00each
Catalog Number: LS-G115393-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CNKSR1 (NM_006314), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CNK | CNK1 | Connector enhancer of KSR 1 | CNKSR1 | CNK homolog protein 1 | Connector enhancer of KSR-like | KSR | HCNK1
Origin Species
Human
NCBI GenBank
NM_006314
SwissProt
Q969H4

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
78.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEPVETWTPG KVATWLRGLD DSLQDYPFED WQLPGKNLLQ LCPQSLEALA VRSLGHQELI
61LGGVEQLQAL SSRLQTENLQ SLTEGLLGAT HDFQSIVQGC LGDCAKTPID VLCAAVELLH
121EADALLFWLS RYLFSHLNDF SACQEIRDLL EELSQVLHED GPAAEKEGTV LRICSHVAGI
181CHNILVCCPK ELLEQKAVLE QVQLDSPLGL EIHTTSNCQH FVSQVDTQVP TDSRLQIQPG
241DEVVQINEQV VVGWPRKNMV RELLREPAGL SLVLKKIPIP ETPPQTPPQV LDSPHQRSPS
301LSLAPLSPRA PSEDVFAFDL SSNPSPGPSP AWTDSASLGP EPLPIPPEPP AILPAGVAGT
361PGLPESPDKS PVGRKKSKGL ATRLSRRRVS CRELGRPDCD GWLLLRKAPG GFMGPRWRRR
421WFVLKGHTLY WYRQPQDEKA EGLINVSNYS LESGHDQKKK YVFQLTHDVY KPFIFAADTL
481TDLSMWVRHL ITCISKYQSP GRAPPPREED CYSETEAEDP DDEAGSHSAS PSPAQAGSPL
541HGDTSPAATP TQRSPRTSFG SLTDSSEEAL EGMVRGLRQG GVSLLGQPQP LTQEQWRSSF
601MRRNRDPQLN ERVHRVRALQ STLKAKLQEL QVLEEVLGDP ELTGEKFRQW KEQNRELYSE
661GLGAWGVAQA EGSSHILTSD STEQSPNSLP SDPEEHSHLC PLTSESSLRP PDL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

713 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.