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CNGA4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CNGA4 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G120504-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CNGA4 (NM_001037329), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CNG2B | CNGA4 | Cgn2 | CNG channel alpha-4 | CNG-4 | CNCA2 | CNG4 | CNG5 | OCNC2 | OCNCBETA | OCNCb | CNGB2
Origin Species
Human
NCBI GenBank
AX465594
SwissProt
Q8IV77

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
65.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSQDTKVKTT ESSPPAPSKA RKLLPVLDPS GDYYYWWLNT MVFPVMYNLI ILVCRACFPD
61LQHGYLVAWL VLDYTSDLLY LLDMVVRFHT GFLEQGILVV DKGRISSRYV RTWSFFLDLA
121SLMPTDVVYV RLGPHTPTLR LNRFLRAPRL FEAFDRTETR TAYPNAFRIA KLMLYIFVVI
181HWNSCLYFAL SRYLGFGRDA WVYPDPAQPG FERLRRQYLY SFYFSTLILT TVGDTPPPAR
241EEEYLFMVGD FLLAVMGFAT IMGSMSSVIY NMNTADAAFY PDHALVKKYM KLQHVNRKLE
301RRVIDWYQHL QINKKMTNEV AILQHLPERL RAEVAVSVHL STLSRVQIFQ NCEASLLEEL
361VLKLQPQTYS PGEYVCRKGD IGQEMYIIRE GQLAVVADDG ITQYAVLGAG LYFGEISIIN
421IKGNMSGNRR TANIKSLGYS DLFCLSKEDL REVLSEYPQA QTIMEEKGRE ILLKMNKLDV
481NAEAAEIALQ EATESRLRGL DQQLDDLQTK FARLLAELES SALKIAYRIE RLEWQTREWP
541MPEDLAEADD EGEPEEGTSK DEEGRASQEG PPGPE
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

575 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.