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CNGA3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CNGA3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CNGA3 antibodies →
$725.00each
Catalog Number: LS-G120144-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CNG3(CNGA3) (NM_001079878), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CCNCa | CCNCalpha | CNGA3 | CCNC1 | CNG channel alpha-3 | CNG3 | ACHM2 | Achromatopsia 2 | MCNG3 | CNCG3 | CNG-3
Origin Species
Human
NCBI GenBank
NM_001298
SwissProt
Q16281

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
76.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAKINTQYSH PSRTHLKVKT SDRDLNRAEN GLSRAHSSSE ETSSVLQPGI AMETRGLADS
61GQGSFTGQGI ARLSRLIFLL RRWAARHVHH QDQGPDSFPD RFRGAELKEV SSQESNAQAN
121VGSQEPADRG RRKKTKKKDA IVVDPSSNLY YRWLTAIALP VFYNWYLLIC RACFDELQSE
181YLMLWLVLDY SADVLYVLDV LVRARTGFLE QGLMVSDTNR LWQHYKTTTQ FKLDVLSLVP
241TDLAYLKVGT NYPEVRFNRL LKFSRLFEFF DRTETRTNYP NMFRIGNLVL YILIIIHWNA
301CIYFAISKFI GFGTDSWVYP NISIPEHGRL SRKYIYSLYW STLTLTTIGE TPPPVKDEEY
361LFVVVDFLVG VLIFATIVGN VGSMISNMNA SRAEFQAKID SIKQYMQFRK VTKDLETRVI
421RWFDYLWANK KTVDEKEVLK SLPDKLKAEI AINVHLDTLK KVRIFQDCEA GLLVELVLKL
481RPTVFSPGDY ICKKGDIGKE MYIINEGKLA VVADDGVTQF VVLSDGSYFG EISILNIKGS
541KSGNRRTANI RSIGYSDLFC LSKDDLMEAL TEYPEAKKAL EEKGRQILMK DNLIDEELAR
601AGADPKDLEE KVEQLGSSLD TLQTRFARLL AEYNATQMKM KQRLSQLESQ VKGGGDKPLA
661DGEVPGDATK TEDKQQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

676 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.