Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CNBD2 / C20orf152 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G107742-100
Catalog Number: LS-G107742
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C20orf152(CNBD2) (NM_080834), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Origin Species
- Human
- NCBI GenBank
- NM_080834
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 66.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MRRHMVTYAW QLLKKELGLY QLAMDIIIMI RVCKMFRQGL RGFREYQIIE TAHWKHPIFS61FWDKKMQSRV TFDTMDFIAE EGHFPPKAIQ IMQKKPSWRT EDEIQAVCNI LQVLDSYRNY121AEPLQLLLAK VMRFERFGRR RVIIKKGQKG NSFYFIYLGT VAITKDEDGS SAFLDPHPKL181LHKGSCFGEM DVLHASVRRS TIVCMEETEF LVVDREDFFA NKLDQEVQKD AQYRFEFFRK241MELFASWSDE KLWQLVAMAK IERFSYGQLI SKDFGESPFI MFISKGSCEV LRLLDLGASP301SYRRWIWQHL ELIDGRPLKT HLSEYSPMER FKEFQIKSYP LQDFSSLKLP HLKKAWGLQG361TSFSRKIRTS GDTLSKMLGP KIQSRPAQSI KCAMINIKPG ELPKEAAVGA YVKVHTVEQG421EILAFLPEGE CDTRPLILMS LGNELIRIRK EIFYELIDND DEMIKKLLKL NIAFPSDEDM481CQKFLQQNSW NIFRKDLLQL LVEPCQSQLF TPNRPKKREI YNPKSVVLDL CSINKTTKPR541YPIFMAPQKY LPPLRIVQAI KAPRYKIREL LATRTRPLEQKLISEEDLAANDILDYKDDDDKV
572 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.