Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CMT2 / MAD2L1BP Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G99259-100
Catalog Number: LS-G99259
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MAD2L1 binding protein(MAD2L1BP) (NM_001003690), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CMT2 | DJ261G23.1 | MAD2L1BP | MAD2L1 binding protein | KIAA0110 | RP1-261G23.6 | MAD2L1-binding protein | Caught by MAD2 protein
- NCBI GenBank
- NM_014628
- SwissProt
- Q15013
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 34.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MARVPLGRSL TLSPRLEHNG MTSAHHNFRL PGSRDSPASA SQVAEIIDLE WYEKSEETHA61SQIELLETSS TQEPLNASEA FCPRDCMVPV VFPGPVSQEG CCQFTCELLK HIMYQRQQLP121LPYEQLKHFY RKPSPQAEEM LKKKPRATTE VSSRKCQQAL AELESVLSHL EDFFARTLVP181RVLILLGGNA LSPKEFYELD LSLLAPYSVD QSLSTAACLR RLFRAIFMAD AFSELQAPPL241MGTVVMAQGH RNCGEDWFRP KLNYRVPSRG HKLTVTLSCG RPSIRTTAWE DYIWFQAPVT301FKGFRETRTRPLEQKLISEEDLAANDILDYKDDDDKV
306 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.