Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CLU1 / KIAA0664 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G113368-100
Catalog Number: LS-G113368
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KIAA0664(CLUH) (NM_015229), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CLU1 | CLUH | KIAA0664
- Origin Species
- Human
- NCBI GenBank
- AB014564
- SwissProt
- O75153
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 146.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MLLNGDCPES LKKEAAAAEP PRENGLDEAG PGDETTGQEV IVIQDTGFSV KILAPGIEPF61SLQVSPQEMV QEIHQVLMDR EDTCHRTCFS LHLDGNVLDH FSELRSVEGL QEGSVLRVVE121EPYTVREARI HVRHVRDLLK SLDPSDAFNG VDCNSLSFLS VFTDGDLGDS GKRKKGLEMD181PIDCTPPEYI LPGSRERPLC PLQPQNRDWK PLQCLKVLTM SGWNPPPGNR KMHGDLMYLF241VITAEDRQVS ITASTRGFYL NQSTAYHFNP KPASPRFLSH SLVELLNQIS PTFKKNFAVL301QKKRVQRHPF ERIATPFQVY SWTAPQAEHA MDCVRAEDAY TSRLGYEEHI PGQTRDWNEE361LQTTRELPRK NLPERLLRER AIFKVHSDFT AAATRGAMAV IDGNVMAINP SEETKMQMFI421WNNIFFSLGF DVRDHYKDFG GDVAAYVAPT NDLNGVRTYN AVDVEGLYTL GTVVVDYRGY481RVTAQSIIPG ILERDQEQSV IYGSIDFGKT VVSHPRYLEL LERTSRPLKI LRHQVLNDRD541EEVELCSSVE CKGIIGNDGR HYILDLLRTF PPDLNFLPVP GEELPEECAR AGFPRAHRHK601LCCLRQELVD AFVEHRYLLF MKLAALQLMQ QNASQLETPS SLENGGPSSL ESKSEDPPGQ661EAGSEEEGSS ASGLAKVKEL AETIAADDGT DPRSREVIRN ACKAVGSISS TAFDIRFNPD721IFSPGVRFPE SCQDEVRDQK QLLKDAAAFL LSCQIPGLVK DCMEHAVLPV DGATLAEVMR781QRGINMRYLG KVLELVLRSP ARHQLDHVFK IGIGELITRS AKHIFKTYLQ GVELSGLSAA841ISHFLNCFLS SYPNPVAHLP ADELVSKKRN KRRKNRPPGA ADNTAWAVMT PQELWKNICQ901EAKNYFDFDL ECETVDQAVE TYGLQKITLL REISLKTGIQ VLLKEYSFDS RHKPAFTEED961VLNIFPVVKH VNPKASDAFH FFQSGQAKVQ QGFLKEGCEL INEALNLFNN VYGAMHVETC1021ACLRLLARLH YIMGDYAEAL SNQQKAVLMS ERVMGTEHPN TIQEYMHLAL YCFASSQLST1081ALSLLYRARY LMLLVFGEDH PEMALLDNNI GLVLHGVMEY DLSLRFLENA LAVSTKYHGP1141KALKVALSHH LVARVYESKA EFRSALQHEK EGYTIYKTQL GEDHEKTKES SEYLKCLTQQ1201AVALQRTMNE IYRNGSSANI PPLKFTAPSM ASVLEQLNVI NGILFIPLSQ KDLENLKAEV1261ARRHQLQEAS RNRDRAEEPM ATEPAPAGAP GDLGSQPPAA KDPSPSVQGSGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
1309 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.