Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CLU / Clusterin Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G129859-100
Catalog Number: LS-G129859
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Clusterin(CLU) (NM_001171138), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- APO-J | Apolipoprotein J | CLU1 | Clusterin | Complement lysis inhibitor | CLI | AAG4 | Aging-associated protein 4 | Ku70-binding protein 1 | KUB1 | SGP2 | APOJ | SGP-2 | Sulfated glycoprotein 2 | CLU | CLU2 | Complement cytolysis inhibitor | NA1/NA2 | SP-40
- Origin Species
- Human
- NCBI GenBank
- NM_001831
- SwissProt
- P10909
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 54.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEACKDSRIG GMMKTLLLFV GLLLTWESGQ VLGDQTVSDN ELQEMSNQGS KYVNKEIQNA61VNGVKQIKTL IEKTNEERKT LLSNLEEAKK KKEDALNETR ESETKLKELP GVCNETMMAL121WEECKPCLKQ TCMKFYARVC RSGSGLVGRQ LEEFLNQSSP FYFWMNGDRI DSLLENDRQQ181THMLDVMQDH FSRASSIIDE LFQDRFFTRE PQDTYHYLPF SLPHRRPHFF FPKSRIVRSL241MPFSPYEPLN FHAMFQPFLE MIHEAQQAMD IHFHSPAFQH PPTEFIREGD DDRTVCREIR301HNSTGCLRMK DQCDKCREIL SVDCSTNNPS QAKLRRELDE SLQVAERLTR KYNELLKSYQ361WKMLNTSSLL EQLNEQFNWV SRLANLTQGE DQYYLRVTTV ASHTSDSDVP SGVTEVVVKL421FDSDPITVTV PVEVSRKNPK FMETVAEKAL QEYRKKHREESGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
460 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.