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CLPB Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CLPB Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CLPB antibodies →
$504.00each
Catalog Number: LS-G109377-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CLPB (NM_030813), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CLPB | SKD3 | HSP78
Origin Species
Human
NCBI GenBank
NM_030813
SwissProt
Q9H078

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
78.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLGSLVLRRK ALAPRLLLRL LRSPTLRGHG GASGRNVTTG SLGEPQWLRV ATGGRPGTSP
61ALFSGRGAAT GGRQGGRFDT KCLAAATWGR LPGPEETLPG QDSWNGVPSR AGLGMCALAA
121ALVVHCYSKS PSNKDAALLE AARANNMQEV SRLLSEGADV NAKHRLGWTA LMVAAINRNN
181SVVQVLLAAG ADPNLGDDFS SVYKTAKEQG IHSLEDGGQD GASRHITNQW TSALEFRRWL
241GLPAGVLITR EDDFNNRLNN RASFKGCTAL HYAVLADDYR TVKELLDGGA NPLQRNEMGH
301TPLDYAREGE VMKLLRTSEA KYQEKQRKRE AEERRRFPLE QRLKEHIIGQ ESAIATVGAA
361IRRKENGWYD EEHPLVFLFL GSSGIGKTEL AKQTAKYMHK DAKKGFIRLD MSEFQERHEV
421AKFIGSPPGY VGHEEGGQLT KKLKQCPNAV VLFDEVDKAH PDVLTIMLQL FDEGRLTDGK
481GKTIDCKDAI FIMTSNVASD EIAQHALQLR QEALEMSRNR IAENLGDVQI SDKITISKNF
541KENVIRPILK AHFRRDEFLG RINEIVYFLP FCHSELIQLV NKELNFWAKR AKQRHNITLL
601WDREVADVLV DGYNVHYGAR SIKHEVERRV VNQLAAAYEQ DLLPGGCTLR ITVEDSDKQL
661LKSPELPSPQ AEKRLPKLRL EIIDKDSKTR RLDIRAPLHP EKVCNTI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

707 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.