Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CLLP / CTNNAL1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G117081-100
Catalog Number: LS-G117081
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CTNNAL1 (NM_003798), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ACRP | Alpha-catulin | Alpha-catenin-related protein | Alpha-CATU | Catenin alpha-like 1 | CLLP | Alpha2-catulin | Catenin alpha-like protein 1 | CTNNAL1
- Origin Species
- Human
- NCBI GenBank
- NM_003798
- SwissProt
- Q9UBT7
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 81.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAASPGPAGV GGAGAVYGSG SSGFALDSGL EIKTRSVEQT LLPLVSQITT LINHKDNTKK61SDKTLQAIQR VGQAVNLAVG RFVKVGEAIA NENWDLKEEI NIACIEAKQA GETIAALTDI121TNLNHLESDG QITIFTDKTG VIKAARLLLS SVTKVLLLAD RVVIKQIITS RNKVLATMER181LEKVNSFQEF VQIFSQFGNE MVEFAHLSGD RQNDLKDEKK KAKMAAARAV LEKCTMMLLT241ASKTCLRHPN CESAHKNKEG VFDRMKVALD KVIEIVTDCK PNGETDISSI SIFTGIKEFK301MNIEALRENL YFQSKENLSV TLEVILERME DFTDSAYTSH EHRERILELS TQARMELQQL361ISVWIQAQSK KTKSIAEELE LSILKISHSL NELKKELHST ATQLAADLLK YHADHVVLKA421LKLTGVEGNL EALAEYACKL SEQKEQLVET CRLLRHISGT EPLEITCIHA EETFQVTGQQ481IISAAETLTL HPSSKIAKEN LDVFCEAWES QISDMSTLLR EINDVFEGRR GEKYGYLSLP541KPMKNNANLK SLKPDKPDSE EQAKIAKLGL KLGLLTSDAD CEIEKWEDQE NEIVQYGRNM601SSMAYSLYLF TRGEGPLKTS QDLIHQLEVF AAEGLKLTSS VQAFSKQLKD DDKLMLLLEI661NKLIPLCHQL QTVTKTSLQN KVFLKVDKCI TKTRSMMALL VQLLSLCYKL LKKLQMENNG721WVSVTNKDTM DSKTTRTRPLEQKLISEEDLAANDILDYKDDDDKV
734 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.