Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CLIP4 / RSNL2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G109842-100
Catalog Number: LS-G109842
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CLIP4 (NM_024692), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CLIP4 | Restin-like 2 | Restin-like protein 2 | RSNL2
- Origin Species
- Human
- NCBI GenBank
- NM_024692
- SwissProt
- Q8N3C7
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 76.1 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MTIEDLPDFP LEGNPLFGRY PFIFSASDTP VIFSISAAPM PSDCEFSFFD PNDASCQEIL61FDPKTSVSEL FAILRQWVPQ VQQNIDIIGN EILKRGCNVN DRDGLTDMTL LHYTCKSGAH121GIGDVETAVK FATQLIDLGA DISLRSRWTN MNALHYAAYF DVPELIRVIL KTSKPKDVDA181TCSDFNFGTA LHIAAYNLCA GAVKCLLEQG ANPAFRNDKG QIPADVVPDP VDMPLEMADA241AATAKEIKQM LLDAVPLSCN ISKAMLPNYD HVTGKAMLTS LGLKLGDRVV IAGQKVGTLR301FCGTTEFASG QWAGIELDEP EGKNNGSVGK VQYFKCAPKY GIFAPLSKIS KAKGRRKNIT361HTPSTKAAVP LIRSQKIDVA HVTSKVNTGL MTSKKDSASE STLSLPPGEE LKTVTEKDVA421LLGSVSSCSS TSSLEHRQSY PKKQNAISSN KKTMSKSPSL SSRASAGLNS SATSTANNSR481CEGELRLGER VLVVGQRLGT IRFFGTTNFA PGYWYGIELE KPHGKNDGSV GGVQYFSCSP541RYGIFAPPSR VQRVTDSLDT LSEISSNKQN HSYPGFRRSF STTSASSQKE INRRNAFSKS601KAALRRSWSS TPTAGGIEGS VKLHEGSQVL LTSSNEMGTV RYVGPTDFAS GIWLGLELRS661AKGKNDGSVG DKRYFTCKPN HGVLVRPSRV TYRGINGSKL VDENCTRTRPLEQKLISEEDLAANDILDYKDDDDKV
705 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.