Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CLEC16A / KIAA0350 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G113369-100
Catalog Number: LS-G113369
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CLEC16A (NM_015226), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- KIAA0350 | CLEC16A | Gop-1 | Protein CLEC16A
- Origin Species
- Human
- NCBI GenBank
- AB002348
- SwissProt
- Q2KHT3
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 117.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MFGRSRSWVG GGHGKTSRNI HSLDHLKYLY HVLTKNTTVT EQNRNLLVET IRSITEILIW61GDQNDSSVFD FFLEKNMFVF FLNILRQKSG RYVCVQLLQT LNILFENISH ETSLYYLLSN121NYVNSIIVHK FDFSDEEIMA YYISFLKTLS LKLNNHTVHF FYNEHTNDFA LYTEAIKFFN181HPESMVRIAV RTITLNVYKV DNQAMLHYIR DKTAVPYFSN LVWFIGSHVI ELDDCVQTDE241EHRNRGKLSD LVAEHLDHLH YLNDILIINC EFLNDVLTDH LLNRLFLPLY VYSLENQDKG301GERPKISLPV SLYLLSQVFL IIHHAPLVNS LAEVILNGDL SEMYAKTEQD IQRSSAKPSI361RCFIKPTETL ERSLEMNKHK GKRRVQKRPN YKNVGEEEDE EKGPTEDAQE DAEKAKGTEG421GSKGIKTSGE SEEIEMVIME RSKLSELAAS TSVQEQNTTD EEKSAAATCS ESTQWSRPFL481DMVYHALDSP DDDYHALFVL CLLYAMSHNK GMDPEKLERI QLPVPNAAEK TTYNHPLAER541LIRIMNNAAQ PDGKIRLATL ELSCLLLKQQ VLMSAGCIMK DVHLACLEGA REESVHLVRH601FYKGEDIFLD MFEDEYRSMT MKPMNVEYLM MDASILLPPT GTPLTGIDFV KRLPCGDVEK661TRRAIRVFFM LRSLSLQLRG EPETQLPLTR EEDLIKTDDV LDLNNSDLIA CTVITKDGGM721VQRFLAVDIY QMSLVEPDVS RLGWGVVKFA GLLQDMQVTG VEDDSRALNI TIHKPASSPH781SKPFPILQAT FIFSDHIRCI IAKQRLAKGR IQARRMKMQR IAALLDLPIQ PTTEVLGFGL841GSSTSTQHLP FRFYDQGRRG SSDPTVQRSV FASVDKVPGF AVAQCINQHS SPSLSSQSPP901SASGSPSGSG STSHCDSGGT SSSSTPSTAQ SPADAPMSPE LPKPHLPDQL VIVNETEADS961KPSKNVARSA AVETASLSPS LVPARQPTIS LLCEDTADTL SVESLTLVPP VDPHSLRSLT1021GMPPLSTPAA ACTEPMGEEA ACAEPVGTAE DTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1051 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.