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CLCN2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CLCN2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G100276-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CLCN2 (NM_004366), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Chloride channel protein 2 | Chloride channel 2 | ClC-2 | CIC-2 | CLC2 | EGI3 | EJM6 | ECA2 | ECA3 | EGMA | EJM8 | CLCN2 | EGI11
Origin Species
Human
NCBI GenBank
NM_004366
SwissProt
P51788

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
98.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAAAAAEEGM EPRALQYEQT LMYGRYTQDL GAFAKEEAAR IRLGGPEPWK GPPSSRAAPE
61LLEYGRSRCA RCRVCSVRCH KFLVSRVGED WIFLVLLGLL MALVSWVIDY AIAACLQAQQ
121WMSRGLNTSI LLQYLAWVTY PVVLITFSAG FTQILAPQAV GSGIPEMKTI LRGVVLKEYL
181TLKTFIAKVI GLTCALGSGM PLGKEGPFVH IASMCAALLS KFLSLFGGIY ENESRNTEML
241AAACAVGVGC CFAAPIGGVL FSIEVTSTFF AVRNYWRGFF AATFSAFIFR VLAVWNRDEE
301TITALFKTRF RLDFPFDLQE LPAFAVIGIA SGFGGALFVY LNRKIVQVMR KQKTINRFLM
361RKRLLFPALV TLLISTLTFP PGFGQFMAGQ LSQKETLVTL FDNRTWVRQG LVEELEPPST
421SQAWNPPRAN VFLTLVIFIL MKFWMSALAT TIPVPCGAFM PVFVIGAAFG RLVGESMAAW
481FPDGIHTDSS TYRIVPGGYA VVAALAGAVT HTVSTAVIVF ELTGQIAHIL PVMIAVILAN
541AVAQSLQPSL YDSIIRIKKL PYLPELGWGR HQQYRVRVED IMVRDVPHVA LSCTFRDLRL
601ALHRTKGRML ALVESPESMI LLGSIERSQV VALLGAQLSP ARRRQHMQER RATQTSPLSD
661QEGPPSPEAS VCFQVNTEDS AFPAARGETH KPLKPALKRG PSVTRNLGES PTGSAESAGI
721ALRSLFCGSP PPEAASEKLE SCEKRKLKRV RISLASDADL EGEMSPEEIL EWEEQQLDEP
781VNFSDCKIDP APFQLVERTS LHKTHTIFSL LGVDHAYVTS IGRLIGIVTL KELRKAIEGS
841VTAQGVKVRP PLASFRDSAT SSSDTETTEV HALWGPHSRH GLPREGSPSD SDDKCQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

896 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.