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CLCC1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CLCC1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G101293-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CLCC1 (NM_015127), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CLCC1 | Chloride channel CLIC-like 1 | KIAA0761 | MCLC | Mid1-related chloride channel | RP11-475E11.6
Origin Species
Human
NCBI GenBank
NM_015127
SwissProt
Q96S66

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
56.1 kda
Protein Type
Lysate
Amino Acid Sequence
1MLCSLLLCEC LLLVAGYAHD DDWIDPTDML NYDAASGTMR KSQAKYGISG EKDVSPDLSC
61ADEISECYHK LDSLTYKIDE CEKKKREDYE SQSNPVFRRY LNKILIEAGK LGLFHDFETW
121KWRFEDSFGV DPYNVLMVLL CLLCIVVLVA TELWTYVRWY TQLRRVLIIS FLFSLGWNWM
181YLYKLAFAQH QAEVAKMEPL NNVCAKKMDW TGSIWEWFRS SWTYKDDPCQ KYYELLLVNP
241IWLVPPTKAL AVTFTTFVTE PLKHIGKGTG EFIKALMKEI PALLHLPVLI IMALAILSFC
301YGAGKSVHVL RHIGGPESEP PQALRPRDRR RQEEIDYRPD GGAGDADFHY RGQMGPTEQG
361PYAKTYEGRR EILRERDVDL RFQTGNKSPE VLRAFDVPDA EAREHPTVVP SHKSPVLDTK
421PKETGGILGE GTPKESSTES SQSAKPVSGQ DTSGNTEGSP AAEKAQLKSE AAGSPDQGST
481YSPARGVAGP RGQDPVSSPC G
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

501 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.