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CLASRP Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CLASRP Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CLASRP antibodies →
$504.00each
Catalog Number: LS-G114886-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CLASRP (NM_007056), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CLASRP | CLASP | SWAP2 | SFRS16
Origin Species
Human
NCBI GenBank
NM_007056
SwissProt
Q8N2M8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
75.21 kDa
Protein Type
Lysate
Amino Acid Sequence
1MVDYKKRAER RREYYEKIKK DPAQFLQVHG RACKVHLDSA VALAAESPVN MMPWQGDTNN
61MIDRFDVRAH LDHIPDYTPP LLTTISPEQE SDERKCNYER YRGLVQNDFA GISEEQCLYQ
121IYIDELYGGL QRPSEDEKKK LAEKKASIGY TYEDSTVAEV EKAAEKPEEE ESAAEEESNS
181DEDEVIPDID VEVDVDELNQ EQVADLNKQA TTYGMADGDF VRMLRKDKEE AEAIKHAKAL
241EEEKAMYSGR RSRRQRREFR EKRLRGRKIS PPSYARRDSP TYDPYKRSPS ESSSESRSRS
301RSPTPGREEK ITFITSFGGS DEEAAAAAAA AAASGVTTGK PPAPPQPGGP APGRNASARR
361RSSSSSSSSS ASRTSSSRSS SRSSSRSRRG GGYYRSGRHA RSRSRSWSRS RSRSRRYSRS
421RSRGRRHSGG GSRDGHRYSR SPARRGGYGP RRRSRSRSHS GDRYRRGGRG LRHHSSSRSR
481SSWSLSPSRS RSLTRSRSHS PSPSQSRSRS RSRSQSPSPS PAREKLTRPA ASPAVGEKLK
541KTEPAAGKET GAAKPKLTPQ EKLKLRMQKA LNRQFKADKK AAQEKMIQQE HERQEREDEL
601RAMARKIRMK ERERREKERE EWERQYSRQS RSPSPRYSRE YSSSRRRSRS RSRSPHYRH
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

659 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.