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CKAP4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CKAP4 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G115060-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CKAP4 (NM_006825), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
63 kDa membrane protein | ERGIC-63 | CKAP4 | CLIMP-63 | p63
Origin Species
Human
NCBI GenBank
NM_006825
SwissProt
Q07065

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
65.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPSAKQRGSK GGHGAASPSE KGAHPSGGAD DVAKKPPPAP QQPPPPPAPH PQQHPQQHPQ
61NQAHGKGGHR GGGGGGGKSS SSSSASAAAA AAAASSSASC SRRLGRALNF LFYLALVAAA
121AFSGWCVHHV LEEVQQVRRS HQDFSRQREE LGQGLQGVEQ KVQSLQATFG TFESILRSSQ
181HKQDLTEKAV KQGESEVSRI SEVLQKLQNE ILKDLSDGIH VVKDARERDF TSLENTVEER
241LTELTKSIND NIAIFTEVQK RSQKEINDMK AKVASLEESE GNKQDLKALK EAVKEIQTSA
301KSREWDMEAL RSTLQTMESD IYTEVRELVS LKQEQQAFKE AADTERLALQ ALTEKLLRSE
361ESVSRLPEEI RRLEEELRQL KSDSHGPKED GGFRHSEAFE ALQQKSQGLD SRLQHVEDGV
421LSMQVASARQ TESLESLLSK SQEHEQRLAA LQGRLEGLGS SEADQDGLAS TVRSLGETQL
481VLYGDVEELK RSVGELPSTV ESLQKVQEQV HTLLSQDQAQ AARLPPQDFL DRLSSLDNLK
541ASVSQVEADL KMLRTAVDSL VAYSVKIETN ENNLESAKGL LDDLRNDLDR LFVKVEKIHE
601KV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

602 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.