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CKAP2L Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CKAP2L Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CKAP2L antibodies →
$504.00each
Catalog Number: LS-G106240-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CKAP2L (NM_152515), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CKAP2L
Origin Species
Human
NCBI GenBank
AA448741

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
83.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MVGPGPTAAA AVEERQRKLQ EYLAAKGKLK SQNTKPYLKS KNNCQNQPPS KSTIRPKNDV
61TNHVVLPVKP KRSISIKLQP RPPNTAGSQK PKLEPPKLLG KRLTSECVSS NPYSKPSSKS
121FQQCEAGSST TGELSRKPVG SLNIEQLKTT KQQLTDQGNG KCIDFMNNIH VENESLDNFL
181KETNKENLLD ILTEPERKPD PKLYTRSKPK TDSYNQTKNS LVPKQALGKS SVNSAVLKDR
241VNKQFVGETQ SRTFPVKSQQ LSRGADLARP GVKPSRTVPS HFIRTLSKVQ SSKKPVVKNI
301KDIKVNRSQY ERPNETKIRS YPVTEQRVKH TKPRTYPSLL QGEYNNRHPN IKQDQKSSQV
361CIPQTSCVLQ KSKAISQRPN LTVGRFNSAI PSTPSIRPNG TSGNKHNNNG FQQKAQTLDS
421KLKKAVPQNH FLNKTAPKTQ ADVTTVNGTQ TNPNIKKKAT AEDRRKQLEE WQKSKGKTYK
481RPPMELKTKR KVIKEMNISF WKSIEKEEEE KKAQLELSSK INNTLTECLN LIEGGVPSNE
541ILNILSSIPE AEKFAKFWIC KAKLLASKGT FDVIGLYEEA IKNGATPIQE LRKVVLNILQ
601DSNRTTEGIT SDSLVAETSI TSVEELAKKM ESVKSCLSPK EREQVTATPR IAKAEQHNYP
661GIKLQIGPIP RINGMPEVQD MKFITPVRRS SRIERAVSRY PEMLQEHDLV VASLDELLEV
721EETKCFIFRR NEALPVTLGF QTPES
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

745 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.