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CILP2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CILP2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G105880-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CILP2 (NM_153221), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CILP-2 | CLIP-2 | CILP2
Origin Species
Human
NCBI GenBank
NM_153221
SwissProt
Q8IUL8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
126.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MASLLPLLCL CVVAAHLAGA RDATPTEEPM ATALGLERRS VYTGQPSPAL EDWEEASEWT
61SWFNVDHPGG DGDFESLAAI RFYYGPARVC PRPLALEART TDWALPSAVG ERVHLNPTRG
121FWCLNREQPR GRRCSNYHVR FRCPLEASWG AWGPWGPCSG SCGPGRRLRR RHCPSPAGDA
181CPGRPLEAQK CVRPRCPGCS LDTCECPDHI LLGSVVTPSG QPLLGARVSL RDQPGTVATS
241DAHGTFRVPG VCADSRANIR AQMDGFSAGE AQAQANGSIS VVTIILDKLE KPYLVKHPES
301RVREAGQNVT FCCKASGTPM PKKYSWFHNG TLLDRRAHGY GAHLELRGLR PDQAGIYHCK
361AWNEAGAVRS GTARLTVLAP GQPACDPRPR EYLIKLPEDC GQPGSGPAYL DVGLCPDTRC
421PSLAGSSPRC GDASSRCCSV RRLERREIHC PGYVLPVKVV AECGCQKCLP PRGLVRGRVV
481AADSGEPLRF ARILLGQEPI GFTAYQGDFT IEVPPSTQRL VVTFVDPSGE FMDAVRVLPF
541DPRGAGVYHE VKAMRKKAPV ILHTSQSNTI PLGELEDEAP LGELVLPSGA FRRADGKPYS
601GPVEARVTFV DPRDLTSAAS APSDLRFVDS DGELAPLRTY GMFSVDLRAP GSAEQLQVGP
661VAVRVAASQI HMPGHVEALK LWSLNPETGL WEEESGFRRE GSSGPRVRRE ERVFLVGNVE
721IRERRLFNLD VPERRRCFVK VRAYANDKFT PSEQVEGVVV TLVNLEPAPG FSANPRAWGR
781FDSAVTGPNG ACLPAFCDAD RPDAYTALVT ATLGGEELEP APSLPRPLPA TVGVTQPYLD
841RLGYRRTDHD DPAFKRNGFR INLAKPRPGD PAEANGPVYP WRSLRECQGA PVTASHFRFA
901RVEADKYEYN VVPFREGTPA SWTGDLLAWW PNPQEFRACF LKVKIQGPQE YMVRSHNAGG
961SHPRTRGQLY GLRDARSVRD PERPGTSAAC VEFKCSGMLF DQRQVDRTLV TIMPQGSCRR
1021VAVNGLLRDY LTRHPPPVPA EDPAAFSMLA PLDPLGHNYG VYTVTDQSPR LAKEIAIGRC
1081FDGSSDGFSR EMKADAGTAV TFQCREPPAG RPSLFQRLLE SPATALGDIR REMSEAAQAQ
1141ARASGPLRTR RGRVRQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1156 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.