Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CIART / C1orf51 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G106891-100
Catalog Number: LS-G106891
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C1orf51(CIART) (NM_144697), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C1orf51 | BC017397
- Origin Species
- Human
- SwissProt
- Q8N365
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 41.3 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MDSPSSVSSY SSYSLSSSFP TSPVNSDFGF PSDSEREDKG AHGPRPDTVG QRGGSRPSPG61PIRCRHRSKV SGNQHTPSHP KQRGSASPMA GSGAKRSRDG ELETSLNTQG CTTEGDLLFA121QKCKELQGFI PPLTDLLNGL KMGRFERGLS SFQQSVAMDR IQRIVGVLQK PQMGERYLGT181LLQVEGMLKT WFPQIAAQKS SLGGGKHQLT KHFPSHHSDS AASSPASPME KMDQTQLGHL241ALKPKQPWHL TQWPAMNLTW IHTTPICNPP LSSPGTISFS HGPLGTGTGI GVILFLQHGV301QPFTHSAPTT PVPPTTASPV IPGEPMKLSG EGPRCYSLPV TLPSDWSYTL SPPSLPTLAR361KMTIGHREQQ RSHPPVAADA HLLNLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
385 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.