Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CHTF18 / RUVBL Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G110460-100
Catalog Number: LS-G110460
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CHTF18 (NM_022092), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C16orf41 | C321D2.2 | C321D2.4 | C321D2.3 (novel protein) | CHTF18 | Ctf18 | HCTF18 | Homolog of yeast CHL12 | C321D2.3 | C321D2.4 (novel protein) | CHL12 | RUVBL
- Origin Species
- Human
- SwissProt
- Q8WVB6
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 107.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEDYEQELCG VEDDFHNQFA AELEVLAELE GASTPSPSGV PLFTAGRPPR TFEEALARGD61AASSPAPAAS VGSSQGGARK RQVDADLQPA GSLPHAPRIK RPRLQVVKRL NFRSEEMEEP121PPPDSSPTDI TPPPSPEDLA ELWGHGVSEA AADVGLTRAS PAARNPVLRR PPILEDYVHV181TSTEGVRAYL VLRADPMAPG VQGSLLHVPW RGGGQLDLLG VSLASLKKQV DGERRERLLQ241EAQKLSDTLH SLRSGEEEAA QPLGAPEEEP TDGQDASSHC LWVDEFAPRH YTELLSDDFT301NRCLLKWLKL WDLVVFGHER PSRKPRPSVE PARVSKEATA PGKWKSHEQV LEEMLEAGLD361PSQRPKQKVA LLCGPPGLGK TTLAHVIARH AGYSVVEMNA SDDRSPEVFR TRIEAATQME421SVLGAGGKPN CLVIDEIDGA PVAAINVLLS ILNRKGPQEV GPQGPAVPSG GGRRRRAEGG481LLMRPIICIC NDQFAPSLRQ LKQQAFLLHF PPTLPSRLVQ RLQEVSLRQG MRADPGVLAA541LCEKTDNDIR ACINTLQFLY SRGQRELSVR DVQATRVGLK DQRRGLFSVW QEVFQLPRAQ601RRRVGQDPAL PADTLLLGDG DAGSLTSASQ RFYRVLHAAA SAGEHEKVVQ GLFDNFLRLR661LRDSSLGAVC VALDWLAFDD LLAGAAHHSQ SFQLLRYPPF LPVAFHVLFA SSHTPRITFP721SSQQEAQNRM SQMRNLIQTL VSGIAPATRS RATPQALLLD ALCLLLDILA PKLRPVSTQL781YSTREKQQLA SLVGTMLAYS LTYRQERTPD GQYIYRLEPN VEELCRFPEL PARKPLTYQT841KQLIAREIEV EKMRRAEASA RVENSPQVDG SPPGLEGLLG GIGEKGVHRP APRNHEQRLE901HIMRRAAREE QPEKDFFGRV VVRSTAVPSA GDTAPEQDSV ERRMGTAVGR SEVWFRFNEG961VSNAVRRSLY IRDLLSGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
975 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.