Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CHSY3 / CSS3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G104975-100
Catalog Number: LS-G104975
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CHSY3 (NM_175856), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Chondroitin synthase-2 | Carbohydrate synthase 2 | Chondroitin sulfate synthase 3 | Chondroitin synthase 2 | CHSY-2 | CHSY2 | CHSY3 | CSS3
- Origin Species
- Human
- SwissProt
- Q70JA7
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 100.1 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MAVRSRRPWM SVALGLVLGF TAASWLIAPR VAELSERKRR GSSLCSYYGR SAAGPRAGAQ61QPLPQPQSRP RQEQSPPPAR QDLQGPPLPE AAPGITSFRS SPWQQPPPLQ QRRRGREPEG121ATGLPGAPAA EGEPEEEDGG AAGQRRDGRP GSSHNGSGDG GAAAPSARPR DFLYVGVMTA181QKYLGSRALA AQRTWARFIP GRVEFFSSQQ PPNAGQPPPP LPVIALPGVD DSYPPQKKSF241MMIKYMHDHY LDKYEWFMRA DDDVYIKGDK LEEFLRSLNS SKPLYLGQTG LGNIEELGKL301GLEPGENFCM GGPGMIFSRE VLRRMVPHIG ECLREMYTTH EDVEVGRCVR RFGGTQCVWS361YEMQQLFHEN YEHNRKGYIQ DLHNSKIHAA ITLHPNKRPA YQYRLHNYML SRKISELRYR421TIQLHRESAL MSKLSNTEVS KEDQQLGVIP SFNHFQPRER NEVIEWEFLT GKLLYSAAEN481QPPRQSLSSI LRTALDDTVL QVMEMINENA KSRGRLIDFK EIQYGYRRVN PMHGVEYILD541LLLLYKRHKG RKLTVPVRRH AYLQQLFSKP FFRETEELDV NSLVESINSE TQSFSFISNS601LKILSSFQGA KEMGGHNEKK VHILVPLIGR YDIFLRFMEN FENMCLIPKQ NVKLVIILFS661RDSGQDSSKH IELIKGYQNK YPKAEMTLIP MKGEFSRGLG LEMASAQFDN DTLLLFCDVD721LIFREDFLQR CRDNTIQGQQ VYYPIIFSQY DPKVTNGGNP PTDDYFIFSK KTGFWRDYGY781GITCIYKSDL LGAGGFDTSI QGWGLEDVDL YNKVILSGLR PFRSQEVGVV HIFHPVHCDP841NLDPKQYKMC LGSKASTFAS TMQLAELWLE KHLGVRYNRT LSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
882 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.