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CHST15 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CHST15 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CHST15 antibodies →
$504.00each
Catalog Number: LS-G112959-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CHST15 (NM_015892), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CHST15 | GALNAC4S-6ST | GALNAC4S6ST | KIAA0598 | HBRAG | RP11-47G11.1 | BRAG
Origin Species
Human
NCBI GenBank
AX281669
SwissProt
Q7LFX5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
64.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MRHCINCCIQ LLPDGAHKQQ VNCQGGPHHG HQACPTCKGE NKILFRVDSK QMNLLAVLEV
61RTEGNENWGG FLRFKKGKRC SLVFGLIIMT LVMASYILSG AHQELLISSP FHYGGFPSNP
121SLMDSENPSD TKEHHHQSSV NNISYMKDYP SIKLIINSIT TRIEFTTRQL PDLEDLKKQE
181LHMFSVIPNK FLPNSKSPCW YEEFSGQNTT DPYLTNSYVL YSKRFRSTFD ALRKAFWGHL
241AHAHGKHFRL RCLPHFYIIG QPKCGTTDLY DRLRLHPEVK FSAIKEPHWW TRKRFGIVRL
301RDGLRDRYPV EDYLDLFDLA AHQIHQGLQA SSAKEQSKMN TIIIGEASAS TMWDNNAWTF
361FYDNSTDGEP PFLTQDFIHA FQPNARLIVM LRDPVERLYS DYLYFASSNK SADDFHEKVT
421EALQLFENCM LDYSLRACVY NNTLNNAMPV RLQVGLYAVY LLDWLSVFDK QQFLILRLED
481HASNVKYTMH KVFQFLNLGP LSEKQEALMT KSPASNARRP EDRNLGPMWP ITQKILRDFY
541RPFNARLAQV LADEAFAWKT T
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

561 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.