Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CHORDC1 / CHP1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G126331-100
Catalog Number: LS-G126331
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CHORDC1 (NM_001144073), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CHORD-containing protein 1 | CHP-1 | CHORDC1 | Protein morgana
- Origin Species
- Human
- NCBI GenBank
- NM_012124
- SwissProt
- Q9UHD1
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 35.1 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MALLCYNRGC GQRFDPETNS DDACTYHPGV PVFHDALKGC TKGRHNSEKP PEPVKPEVKT61TEKKELCELK PKFQEHIIQA PKPVEAIKRP SPDEPMTNLE LKISASLKQA LDKLKLSSGN121EENKKEEDND EIKIGTSCKN GGCSKTYQGL ESLEEVCVYH SGVPIFHEGM KYWSCCRRKT181SDFNTFLAQE GCTKGKHMWT KKDAGKKVVP CRHDWHQTGG EVTISVYAKN SLPELSRVEA241NSTLLNVHIV FEGEKEFDQN VKLWGVIDVK RSYVTMTATK IEITMRKAEP MQWASLELPA301AKKQEKQKDA TTDTRTRPLEQKLISEEDLAANDILDYKDDDDKV
313 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.