LSBio
CHFR Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CHFR Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CHFR antibodies →
$504.00each
Catalog Number: LS-G128242-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CHFR (NM_001161347), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CHFR | RING finger protein 196 | RNF196 | RNF116
Origin Species
Human
NCBI GenBank
NM_018223

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
63.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MERPEEGKQS PPPQPWGRLL RLGAEEGEPH VLLRKREWTI GRRRGCDLSF PSNKLVSGDH
61CRIVVDEKSG QVTLEDTSTS GTVINKLKVV KKQTCPLQTG DVIYLVYRKN EPEHRSGGGG
121ISPKGSGPSV ASDEVSSFAS ALPDRKTASF SSLEPQDQED LEPVKKKMRG DGDLDLNGQL
181LVAQPRRNAQ TVHEDVRAAA GKPDKMEETL TCIICQDLLH DCVSLQPCMH TFCAACYSGW
241MERSSLCPTC RCPVERICKN HILNNLVEAY LIQHPDKSRS EEDVQSMDAR NKITQDMLQP
301KVRRSFSDEE GSSEDLLELS DVDSESSDIS QPYVVCRQCP EYRRQAAQPP HCPAPEGEPG
361APQALGDAPS TSVSLTTAVQ DYVCPLQGSH ALCTCCFQPM PDRRAEREQD PRVAPQQCAV
421CLQPFCHLYW GCTRTGCYGC LAPFCELNLG DKCLDGVLNN NSYESDILKN YLATRGLTWK
481NMLTESLVAL QRGVFLLSDY RVTGDTVLCY CCGLRSFREL TYQYRQNIPA SELPVAVTSR
541PDCYWGRNCR TQVKAHHAMK FNHICEQTRF KN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

572 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.