LSBio
CHERP Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CHERP Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CHERP antibodies →
$504.00each
Catalog Number: LS-G100803-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CHERP (NM_006387), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DAN16 | ERPROT 213-21 | ERPROT213-21 | SCAF6 | CHERP | DAN26
Origin Species
Human
NCBI GenBank
NM_006387
SwissProt
Q8IWX8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
99.99 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTMEKQKDNP KFSFLFGGEF YSYYKCKLAL EQQQLICKQQ TPELEPAATM PPLPQPPLAP
61AAPIPPAQGA PSMDELIQQS QWNLQQQEQH LLALRQEQVT AAVAHAVEQQ MQKLLEETQL
121DMNEFDNLLQ PIIDTCTKDA ISAGKNWMFS NAKSPPHCEL MAGHLRNRIT ADGAHFELRL
181HLIYLINDVL HHCQRKQARE LLAALQKVVV PIYCTSFLAV EEDKQQKIAR LLQLWEKNGY
241FDDSIIQQLQ SPALGLGQYQ ATLINEYSSV VQPVQLAFQQ QIQTLKTQHE EFVTSLAQQQ
301QQQQQQQQQL QMPQMEAEVK ATPPPPAPPP APAPAPAIPP TTQPDDSKPP IQMPGSSEYE
361APGGVQDPAA AGPRGPGPHD QIPPNKPPWF DQPHPVAPWG QQQPPEQPPY PHHQGGPPHC
421PPWNNSHEGM WGEQRGDPGW NGQRDAPWNN QPDAAWNSQF EGPWNSQHEQ PPWGGGQREP
481PFRMQRPPHF RGPFPPHQQH PQFNQPPHPH NFNRFPPRFM QDDFPPRHPF ERPPYPHRFD
541YPQGDFPAEM GPPHHHPGHR MPHPGINEHP PWAGPQHPDF GPPPHGFNGQ PPHMRRQGPP
601HINHDDPSLV PNVPYFDLPA GLMAPLVKLE DHEYKPLDPK DIRLPPPMPP SERLLAAVEA
661FYSPPSHDRP RNSEGWEQNG LYEFFRAKMR ARRRKGQEKR NSGPSRSRSR SKSRGRSSSR
721SNSRSSKSSG SYSRSRSRSC SRSYSRSRSR SRSRSRSSRS RSRSQSRSRS KSYSPGRRRR
781SRSRSPTPPS SAGLGSNSAP PIPDSRLGEE NKGHQMLVKM GWSGSGGLGA KEQGIQDPIK
841GGDVRDKWDQ YKGVGVALDD PYENYRRNKS YSFIARMKAR DECK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

884 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.