Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CHEMR23 / CMKLR1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G116894-100
Catalog Number: LS-G116894
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CMKLR1 (NM_004072), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CMKLR1 | Chemokine receptor-like 1 | DEZ | CHEMERINR | Resolvin E1 receptor | RVER1 | Chemokine-like receptor 1 | ChemR23 | G-protein coupled receptor DEZ
- Origin Species
- Human
- NCBI GenBank
- NM_004072
- SwissProt
- Q99788
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 41.9 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MEDEDYNTSI SYGDEYPDYL DSIVVLEDLS PLEARVTRIF LVVVYSIVCF LGILGNGLVI61IIATFKMKKT VNMVWFLNLA VADFLFNVFL PIHITYAAMD YHWVFGTAMC KISNFLLIHN121MFTSVFLLTI ISSDRCISVL LPVWSQNHRS VRLAYMACMV IWVLAFFLSS PSLVFRDTAN181LHGKISCFNN FSLSTPGSSS WPTHSQMDPV GYSRHMVVTV TRFLCGFLVP VLIITACYLT241IVCKLQRNRL AKTKKPFKII VTIIITFFLC WCPYHTLNLL ELHHTAMPGS VFSLGLPLAT301ALAIANSCMN PILYVFMGQD FKKFKVALFS RLVNALSEDT GHSSYPSHRS FTKMSSMNER361TSMNERETGM LTRTRPLEQKLISEEDLAANDILDYKDDDDKV
371 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.