LSBio
CFAP69 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CFAP69 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CFAP69 antibodies →
$725.00each
Catalog Number: LS-G128393-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C7orf63(CFAP69) (NM_001160138), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C7orf63
Origin Species
Human
NCBI GenBank
NM_001039706
SwissProt
A5D8W1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
103.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MWTEEAGATA EAQESGIRNK SSSSSQIPVV GVVTEDDEAQ DVFKPMDLNR VIKLLEETDK
61DGLEEKQLKF VKKLVQCYQN GLPLRDLAQI FKILNLCSGK IKNQPRFIES AYDIIKLCGL
121PFLKKKVSDE ITYAEDTANS IALLGDLMKI PSSELRIQIC YQQASSSYKI QMAEVGGLAK
181TMVQSMTLLE NQLVEKLWVL KVLQHLSTSE VNCTIMMKAQ AASGICTHLN DPDPSGQLLF
241RSSEILWNLL EKSSKEEVIQ QLSNLECLLA LKEVFKNLFM RGFSHYDRQL RNDILVITTI
301IAQNPEAPMI ECGFTKDLIL FATFNEVKSQ NLLVKGLKLS NSYEDFELKK LLFNVIVILC
361KDLPTVQLLI DGKVILALFT YVKKPEKQKI IDWSAAQHEE LQLHAIATLS SVAPLLIEEY
421MSCQGNARVL AFLEWCESED PFFSHGNSFH GTGGRGNKFA QMRYSLRLLR AVVYLEDETV
481NKDLCEKGTI QQMIGIFKNI ISKPNEKEEA IVLEIQSDIL LILSGLCENH IQRKEIFGTE
541GVDIVLHVMK TDPRKLQSGL GYNVLLFSTL DSIWCCILGC YPSEDYFLEK EGIFLLLDLL
601ALNQKKFCNL ILGIMVEFCD NPKTAAHVNA WQGKKDQTAA SLLIKLWRKE EKELGVKRDK
661NGKIIDTKKP LFTSFQEEQK IIPLPANCPS IAVMDVSENI RAKIYAILGK LDFENLPGLS
721AEDFVTLCII HRYLDFKIGE IWNEIYEEIK LEKLRPVTTD KKALEAITTA SENIGKMVAS
781LQSDIIESQA CQDMQNEQKV YAKIQATHKQ RELANKSWED FLARTSNAKT LKKAKSLQEK
841AIEASRYHKR PQNAIFHQTH IKGLNTTVPS GGVVTVESTP ARLVGGPLVD TDIALKKLPI
901RGGALQRVKA VKIVDAPKKS IPT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

923 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.