Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CFAP61 / C20orf26 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G113109-100
Catalog Number: LS-G113109
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C20orf26(CFAP61) (NM_015585), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Origin Species
- Human
- NCBI GenBank
- NM_015585
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 141.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSVLTSPRGK VEVVHCRRTE SQDVYCIKSL IRKFTCKLFG KLNIIYLLEK ANLAVTLCND61KEEIMAQATF LDYRNWNVAK QDDWVSVFRE LDSDIPCTPL NTLFMHLFVA VDEYSVGCCK121EILRTVYKAV PELHFIFLIV PSYMSLGSTL ITVFDQVGNI PCLTYEEDFA VHICHRHSHY181PQLHVRKARV EDHDDLMPIF MRYDTILKET YGEYFLAELI EAQDEENHAV VCEVEGTAVG241FMSVCSRVNM QLLHECFDLG PFHGLCFPHP DDVLESPQDL SVRRSQDAEL RSSSQGSQKI301VEELQEPVSP DTMENIQGNI AREAASEEAL TAVQSGNVSE PEDIEKLSDI STGYAQYHHV361SSRSLASLIL PEEPVHFRPI YRGASAAFCI QLFCIDEKYE ARSLDFMNFV FSLFSDKNFC421VISLPHLTPE FFLIQNFVKM VPFNTCTLEQ DLYVFHRAGL LKSINIRFAT LLDTPGVENL481VSTLMLNKSI LEDLDRYNKA RKDPDGTLLQ AFVAEVAEQI VGIAVIRNEM DIEYIRSHYN541IEDFIYFSHH QREEHGHMHH FALNPIFRHY TKFFLKEILR LGFKSCLYYR VYPKSREGKF601QNPYAHSLTS ALHYLVPVRP RRQIVYPLEK LGINAPSKAV SKDPMSYALN HTNRKLTLEP661KITVNAKIIV VGASSVGISF LETLVFCSHM KFNNLTLIST HGLPGKKLLD TEQRKFLASD721HCFNDKDYAL MSLCSWVNVV VGRMTGIDRA AKHVVLSTDE IVPYDHLILC TGQQYQVPCP781TEADISQHLT NREVPNSSQR RYTGKVPCNH FTLNEEEDCF KALIWIRNNS ITTEGNIIVY841GNTIDTYTTV ETLLNLGVSG SRIHLVQPPP ASTITCINNY SVESAVADAL GAAGVTMYRD901AILAQWNDGL HPDPIYSASF TTPTKPFRLQ CSMFFSFCEK NVDYETFKAL NDACLVYDSR961LVIDTNFHTN DIAIRAAGSL TKFSNRYYSN EWTHSNFSSK EIGFQLAAAM LHLFDPTLEP1021VTEPPANLDR LIPMYKGAKI QGGILPGSYH YLHIAKPAIP TPLEVQMAQP NYGLELVTGS1081AKNGTYFRIH INKYKMVETI TCLSREPFPA SNYIRLFGQH EQLLNNLCAR YDENLITDLY1141SYFTEPWCLA LFHDRFIDLR KELRQILASK EEEDLPSIEQ LAHQIEDEEI NPTEKPRQYL1201KRVFEESIYK TLVERSTLDY LHYNRYHLPM YAWPGIVSGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
1237 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.