Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CFAP58 / CCDC147 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G121864-100
Catalog Number: LS-G121864
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CCDC147(CFAP58) (NM_001008723), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BA127L20.4 | BA127L20.5 (novel protein) | BA554P13.1 | BA127L20.4 (novel protein) | BA127L20.5 | C10orf80 | RP11-554P13.1 | CCDC147
- Origin Species
- Human
- NCBI GenBank
- NM_001008723
- SwissProt
- Q5T655
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 103.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAEEKGGKQV LEESAFEEME RDFQGVLHEL SGDKSLEKFR IEYERLHAVM KKSYDNEKRL61MAKCRELNAE IVVNSAKVAT ALKLSQDDQT TIASLKKEIE KAWKMVDSAY DKEQKAKETI121LALKEEIVNL TKLVEQGSGL SMDQHSNIRD LLRFKEEVTK ERDQLLSEVV KLRESLAQTT181EQQQETERSK EEAEHAISQF QQEIQQRQNE ASREFRKKEK LEKELKQIQA DMDSRQTEIK241ALQQYVQKSK EELQKLEQQL KEQKILNERA AKELEQFQMR NAKLQQENEQ HSLVCEQLSQ301ENQQKALELK AKEEEVHQMR LDIGKLNKIR EQIHKKLHHT EDQKAEVEQH KETLKNQIVG361LEREVEASKK QAELDRKAMD ELLRERDILN KNMLKAVNAT QKQTDLVKLH EQAKRNLEGE421IQNYKDEAQK QRKIIFHLEK ERDRYINQAS DLTQKVLMNM EDIKVRETQI FDYRKKIAES481EIKLKQQQNL YEAVRSDRNL YSKNLVEAQD EITDMKRKLK IMIHQVDELK EDISAKESAL541VKLHLEQQRI EKEKETLKAE LQKLRQQALE TKHFIEKQEA EERKLLRIIA EADGERLRQK601KELDQVISER DILGSQLVRR NDELALLYEK IKIQQSVLNK GESQYNQRLE DMRILRLEIK661KLRREKGILA RSMANVEELR QEFFHMQREL LKERTRCRAL EEELENPLNV HRWRKLEASD721PNAYELIQKI HTLQKRLISK TEEVVEKELL LQEKEKLYME LKHVLARQPG PEAAEQLKLY781RRTLHDKKQQ LKVLSSELNM YEVQSKEYKY EVEKLTNELQ NLKKKYLAQK RKEQLQKNKD841TAPMDNTFLM VKPNGPGFTG GGFPLRSTKM TFTRTRPLEQKLISEEDLAANDILDYKDDDDKV
872 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.