Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CFAP53 / CCDC11 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G106796-100
Catalog Number: LS-G106796
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CCDC11(CFAP53) (NM_145020), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- HTX6 | CCDC11
- Origin Species
- Human
- SwissProt
- Q96M91
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 61.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MYSQRFGTVQ REVKGPTPKV VIVRSKPPKG QGAEHHLERI RRSHQKHNAI LASIKSSERD61RLKAEWDQHN DCKILDSLVR ARIKDAVQGF IINIEERRNK LRELLALEEN EYFTEMQLKK121ETIEEKKDRM REKTKLLKEK NEKERQDFVA EKLDQQFRER CEELRVELLS IHQKKVCEER181KAQIAFNEEL SRQKLVEEQM FSKLWEEDRL AKEKREAQEA RRQKELMENT RLGLNAQITS241IKAQRQATQL LKEEEARLVE SNNAQIKHEN EQDMLKKQKA KQETRTILQK ALQERIEHIQ301QEYRDEQDLN MKLVQRALQD LQEEADKKKQ KREDMIREQK IYHKYLAQRR EEEKAQEKEF361DRILEEDKAK KLAEKDKELR LEKEARRQLV DEVMCTRKLQ VQEKLQREAK EQEERAMEQK421HINESLKELN CEEKENFARR QRLAQEYRKQ LQMQIAYQQQ SQEAEKEEKR REFEAGVAAN481KMCLDKVQEV LSTHQVLPQN IHPMRKACPS KLPPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
514 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.