Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CFAP45 / CCDC19 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G114496-100
Catalog Number: LS-G114496
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CCDC19(CFAP45) (NM_012337), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CCDC19 | NESG1 | RP11-190A12.6
- Origin Species
- Human
- NCBI GenBank
- NM_012337
- SwissProt
- Q9UL16
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 65.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MPLSTAGILS SSSAASNRSR NKARYRTKAV SSEVDESLFG DIKSPAQGQS DSPIVLLRDK61HTLQKTLTAL GLDRKPETIQ LITRDMVREL IVPTEDPSGE SLIISPEEFE RIKWASHVLT121REELEARDQA FKKEKEATMD AVMTRKKIMK QKEMVWNNNK KLSDLEEVAK ERAQNLLQRA181NKLRMEQEEE LKDMSKIILN AKCHAIRDAQ ILEKQQIQKE LDTEEKRLDQ MMEVERQKSI241QRQEELERKR REERIRGRRQ IVEQMEKNQE ERSLLAEQRE QEKEQMLEYM EQLQEEDLKD301MERRQQQKLK MQAEIKRIND ENQKQKAELL AQEKLADQMV MEFTKKKMAR EAEFEAEQER361IRREKEKEIA RLRAMQEKAQ DYQAEQDALR AKRNQEVADR EWRRKEKENA RKKMETEAEL421RKSRLEQVAF KEHALAVQVQ RDRDEFERIL RAQREQIEKE RLEEEKKATG RLQHANELRR481QVRENQQKEV QNRIATFEEG RRLKEEAQKR RERIDEIKRK KLEELRATGL PEKYCIEAER541KANILPATSV NTRTRPLEQKLISEEDLAANDILDYKDDDDKV
551 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.