Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CFAP44 / WDR52 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G111808-100
Catalog Number: LS-G111808
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human WDR52(CFAP44) (NM_018338), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- WDR52 | WD repeat domain 52
- Origin Species
- Human
- NCBI GenBank
- NM_018338
- SwissProt
- Q96MT7
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 111.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MKEPDDQDTD GEKSVTSKSD GKKSLRSSKS ESRSPVQEDN TFLEDDTDET FTKGEGSYLE61EDSDEERLEG SLSSFQYGDL QSTTVPQQTP APAVEEAEEE VKKKISESFF YDYMELASMP121FVTLDSNIPL DLLTLVHSFG YDCRKRANLQ LLDDSIAIYI AGNQLIFLNL KTKEQIYLRS181SSGEGIGVIG VHPHKTYFTV AEKGSFPDII IYEYPSLRPY RVLRDGTEKG YAYVDFNYSG241NLLASVGSNP DYTLTIWNWK EEQPILRTKA FSQEVFKVTF NPDKEEQLTT SGSGHIKFWE301MAFTFTGLKL QGSLGRFGKT ITTDIEGYME LPDGKVLSGS EWGNMLLWEG GLIKVELCRG361TSKSCHNGPI NQIMLYEGEV ITVGSDGYVR IWDFETIDTA DVIDETGLLE IEPINELQVD421KNVNLFSMIK MNETGNNFWL AQDANGAIWK LDLSFSNITQ DPECLFSFHS GAIEAVAVSP481LTYLMATTAL DCSVRIYDFA SKTPLAQMKF KQGGTALVWV PRMVNFTGAQ IIVGFEDGVV541RILELYDPKG LTIFAGRKKI LDADIQLKQV FKPHTACVTA LAYERDGEIL ATGSKDQTVF601FFEVERDYKP IGYINTPGPV CQLMWSPMSH PESTLLIICE NGYILEAPLP TIKQEEDDHD661VVSYEIKDMC IKCFHFSSVK SKILRLIEIE KRERQRELKE KIREERRNKL AAEMGEDGEK721EFQEEEEEKE EEEEEEEPLP EIFIPSTPSP ILCGFYSEPG KFWVSLGGYD SGFLYHCEFP781PCDESSDFKE QKDEPIDVRY LADTEDNPIQ TITFNINKVM MFCGMKNGAI RVYVLNQNDP841SLTSLVDYWH FNMHDNNYGC IKSIANSFDD RFLVTAGADG NIFVFNIFSE FMLRKDMKAK901VPSPRFGIET EPIPEDIEDP KAYSIENARR KREHDKLMKE VGEIKARKRE QIKALRSEFC961NLLEMNEKLP KHMQFKRTVS LITRTRPLEQKLISEEDLAANDILDYKDDDDKV
982 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.