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CERKL Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CERKL Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G128197-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CERKL (NM_001160277), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Ceramide kinase-like | Ceramide kinase-like protein | RP26 | CERKL
Origin Species
Human
SwissProt
Q49MI3

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
57.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPWRRRRNRV SALEGGREEE APPEAAAVPP ALLTSPQQTE AAAERILLRG IFEIGRDSCD
61VVLSERALRW RPIQPERPAG DSKYDLLCKE EFIELKDIFS VKLKRRCSVK QQRSGTLLGI
121TLFICLKKEQ NKLKNSTLDL INLSEDHCDI WFRQFKKILA VMEYEGHALS LLKECELQGF
181DGGHRKPLFA IHWSVQRLFT GMQTLEPSVV CVGGDGSASE VAHALLLRAQ KNAGMETDRI
241LTPVRAQLPL GLIPAGSTNV LAHSLHGVPH VITATLHIIM GHVQLVDVCT FSTAGKLLRF
301GFSAMFGFGG RTLALAEKYR WMSPNQRRDF AVVKALAKLK AEDCEISFLP FNSSDDVQER
361RAQGSPKSDC NDQWQMIQGQ FLNVSIMAIP CLCSVAPRGL APNTRLNNGS MALIIARNTS
421RPEFIKHLKR YASVKNQFNF PFVETYTVEE VKVHPRNNTG GYNPEEEEDE TASENCFPWN
481VDGDLMEVAS EVHIRLHPRL ISLYGGSMEE MIPK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

514 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.