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CERCAM Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CERCAM Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CERCAM antibodies →
$504.00each
Catalog Number: LS-G129058-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CEECAM1(CERCAM) (NM_016174), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CEECAM1 | CERCAM | GLT25D3 | KIAA1502
Origin Species
Human
NCBI GenBank
NM_016174
SwissProt
Q5T4B2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
67.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MRAARAAPLL QLLLLLGPWL EAAGVAESPL PAVVLAILAR NAEHSLPHYL GALERLDYPR
61ARMALWCATD HNVDNTTEML QEWLAAVGDD YAAVVWRPEG EPRFYPDEEG PKHWTKERHQ
121FLMELKQEAL TFARNWGADY ILFADTDNIL TNNQTLRLLM GQGLPVVAPM LDSQTYYSNF
181WCGITPQGYY RRTAEYFPTK NRQRRGCFRV PMVHSTFLAS LRAEGADQLA FYPPHPNYTW
241PFDDIIVFAY ACQAAGVSVH VCNEHRYGYM NVPVKSHQGL EDERVNFIHL ILEALVDGPR
301MQASAHVTRP SKRPSKIGFD EVFVISLARR PDRRERMLAS LWEMEISGRV VDAVDGWMLN
361SSAIRNLGVD LLPGYQDPYS GRTLTKGEVG CFLSHYSIWE EVVARGLARV LVFEDDVRFE
421SNFRGRLERL MEDVEAEKLS WDLIYLGRKQ VNPEKETAVE GLPGLVVAGY SYWTLAYALR
481LAGARKLLAS QPLRRMLPVD EFLPIMFDQH PNEQYKAHFW PRDLVAFSAQ PLLAAPTHYA
541GDAEWLSDTE TSSPWDDDSG RLISWSGSQK TLRSPRLDLT GSSGHSLQPQ PRDEL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

595 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.