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CEP97 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CEP97 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G109935-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CEP97 (NM_024548), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CEP97 | Centrosomal protein of 97 kDa | Centrosomal protein 97kDa | LRRIQ2
Origin Species
Human
NCBI GenBank
NM_024548

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
96.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAVARVDAAL PPGEGSVVNW SGQGLQKLGP NLPCEADIHT LILDKNQIIK LENLEKCKRL
61IQLSVANNRL VRMMGVAKLT LLRVLNLPHN SIGCVEGLKE LVHLEWLNLA GNNLKAMEQI
121NSCTALQHLD LSDNNISQIG DLSKLVSLKT LLLHGNIITS LRMAPAYLPR SLAILSLAEN
181EIRDLNEISF LASLTELEQL SIMNNPCVMA TPSIPGFDYR PYIVSWCLNL RVLDGYVISQ
241KESLKAEWLY SQGKGRAYRP GQHIQLVQYL ATVCPLTSTL GLQTAEDAKL EKILSKQRFH
301QRQLMNQSQN EELSPLVPVE TRASLIPEHS SPVQDCQISQ ESEPVIQVNS WVGINSNDDQ
361LFAVKNNFPA SVHTTRYSRN DLHLEDIQTD EDKLNCSLLS SESTFMPVAS GLSPLSPTVE
421LRLQGINLGL EDDGVADESV KGLESQVLDK EEEQPLWAAN ENSVQMMRSE INTEVNEKAG
481LLPCPEPTII SAILKDDNHS LTFFPESTEQ KQSDIKKPEN TQPENKETIS QATSEKLPMI
541LTQRSVALGQ DKVALQKLND AATKLQACWR GFYARNYNPQ AKDVRYEIRL RRMQEHIVCL
601TDEIRRLRKE RDEERIKKFV QEEAFRFLWN QVRSLQVWQQ TVDQRLSSWH TDVPPISSTL
661VPSKHPLFTQ SQESSCDQNA DWFIASDVAP QEKSLPEFPD SGFHSSLTEQ VHSLQHSLDF
721EKSSTEGSES SIMGNSIDTV RYGKESDLGD VSEEHGEWNK ESSNNEQDNS LLEQYLTSVQ
781QLEDADERTN FDTETRDSKL HIACFPVQLD TLSDGASVDE SHGISPPLQG EISQTQENSK
841LNAEVQGQQP ECDSTFQLLH VGVTV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

865 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.