Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CEP85 / CCDC21 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G110254-100
Catalog Number: LS-G110254
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CCDC21(CEP85) (NM_022778), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Centrosomal protein 85kDa | CEP85 | CCDC21 | DKFZP434L0117 | Centrosomal protein of 85 kDa
- Origin Species
- Human
- NCBI GenBank
- NM_022778
- SwissProt
- Q6P2H3
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 85.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAMQEKYPTE GISHVTSPSS DVIQKGSSLG TEWQTPVISE PFRSRFSRCS SVADSGDTAI61GTSCSDIAED FCSSSGSPPF QPIKSHVTIP TAHVMPSTLG TSPAKPNSTP VGPSSSKLPL121SGLAESVGMT RNGDLGAMKH SPGLSRDLMY FSGATGENGI EQSWFPAVGH ERQEEARKFD181IPSMESTLNQ SAMMETLYSD PHHRVRFHNP RTSTSKELYR VLPEAKKAPG SGAVFERNGP241HSNSSGVLPL GLQPAPGLSK PLPSQVWQPS PDTWHPREQS CELSTCRQQL ELIRLQMEQM301QLQNGAICHH PAAFGPSLPI LEPAQWISIL NSNEHLLKEK ELLIDKQRKH ISQLEQKVRE361SELQVHSALL GRPAPFGDVC LLRLQELQRE NTFLRAQFAQ KTEALSREKI DLEKKLSASE421VEVQLIRESL KVALQKHSEE VKKQEERVKG RDKHINNLKK KCQKESEQNR EKQQRIETLE481RYLADLPTLE DHQKQSQQLK DSELKSTELQ EKVTELESLL EETQAICREK EIQLESLRQR541EAEFSSAGHS LQDKQSVEET SGEGPEVEME SWQKRYDSLQ KIVEKQQQKM DQLRSQVQSL601EQEVAQEEGT SQALREEAQR RDSALQQLRT AVKELSVQNQ DLIEKNLTLQ EHLRQAQPGS661PPSPDTAQLA LELHQELASC LQDLQAVCSI VTQRAQGHDP NLSLLLGIHS AQHPETQLDL721QKPDVIKRKL EEVQQLRRDI EDLRTTMSDR YAQDMGENCV TQTRTRPLEQKLISEEDLAANDILDYKDDDDKV
762 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.