Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CEP83 / CCDC41 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G119486-100
Catalog Number: LS-G119486
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CCDC41(CEP83) (NM_001042399), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CCDC41 | NY-REN-58 | NY-REN-58 antigen
- Origin Species
- Human
- NCBI GenBank
- NM_016122
- SwissProt
- Q9Y592
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 82.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MVVSTFTDMD TFPNNFPPGG DSGLTGSQSE FQKMLIDERL RCEHHKANYQ TLKAEHTRLQ61NEHVKLQNEL KHLFNEKQTQ QEKLQLLLEE LRGELVEKTK DLEEMKLQIL TPQKLELLRA121QIQQELETPM RERFRNLDEE VEKYRAVYNK LRYEHTFLKS EFEHQKEEYA RILDEGKIKY181ESEIARLEED KEELRNQLLN VDLTKDSKRV EQLAREKVYL CQKLKGLEAE VAELKAEKEN241SEAQVENAQR IQVRQLAEMQ ATVRSLEAEK QSANLRAERL EKELQSSSEQ NTFLINKLHK301AEREINTLSS KVKELKHSNK LEITDIKLET ARAKSELERE RNKIQSELDG LQSDNEILKA361AVEHHKVLLV EKDRELIRKV QAAKEEGYQK LVVLQDEKLE LENRLADLEK MKVEHDVWRQ421SEKDQYEEKL RASQMAEEIT RKELQSVRLK LQQQIVTIEN AEKEKNENSD LKQQISSLQI481QVTSLAQSEN DLLNSNQMLK EMVERLKQEC RNFRSQAEKA QLEAEKTLEE KQIQWLEEKH541KLHERITDRE EKYNQAKEKL QRAAIAQKKR KSLHENKLKR LQEKVEVLEA KKEELETENQ601VLNRQNVPFE DYTRLQKRLK DIQRRHNEFR SLILVPNMPP TASINPVSFQ SSAMVPSMEL661PFPPHMQEEQ HQRELSLLRK RLEELETTQR KQLEELGSSG ETRTRPLEQKLISEEDLAANDILDYKDDDDKV
701 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.