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CEP76 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CEP76 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G109691-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CEP76 (NM_024899), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C18orf9 | CEP76 | Centrosomal protein of 76 kDa | HsT1705 | Centrosomal protein 76kDa
Origin Species
Human
NCBI GenBank
NM_024899
SwissProt
Q8TAP6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
74.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSLPPEKASE LKQLIHQQLS KMDVHGRIRE ILAETIREEL APDQQHLSTE DLIKALRRRG
61IIDDVMKELN FVTDSVEQEL PSSPKQPICF DRQSTLKKTN IDPTRRYLYL QVLGGKAFLE
121HLQEPEPLPG QVCSTFTLCL HYRNQRFRSK PVPCACEPDF HDGFLLEVHR ESLGDGTRMA
181DSTTMLSISD PIHMVLIKTD IFGETTLVAS YFLEWRSVLG SENGVTSLTV ELMGVGTESK
241VSVGILNIKL EMYPPLNQTL SQEVVNTQLA LERQKTAEKE RLFLVYAKQW WREYLQIRPS
301HNSRLVKIFA QDENGINRPV CSYVKPLRAG RLLDTPRQAA RFVNVLGYER APVIGGGGKQ
361EQWCTLLAFL CRNKGDCEDH ANLLCSLLLG YGLEAFVCVG TKAKGVPHAW VMTCGTDGAI
421TFWESLTGHR YIHKPTNPDE PPVAEQPKPL YPYRTIGCVF NHQMFLGNCQ PSDAVETCVF
481DLNDESKWKP MSEEAIKSVC APGATTSLPP FPPLCASTID ASVTSNEIEM QLRLLVSEHR
541KDLGLTTVWE DQLSYLLSPA LASYEFERTT SISAGNEEFQ DAIRRAVPDG HTFKGFPIHF
601VYRNARRAFA TCLRSPFCEE IICCRGDQVR LAVRVRVFTY PESACAVWIM FACKYRSVL
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

659 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.