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CEP72 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CEP72 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CEP72 antibodies →
$504.00each
Catalog Number: LS-G111969-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CEP72 (NM_018140), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Centrosomal protein 72kDa | CEP72 | Centrosomal protein of 72 kDa | KIAA1519
Origin Species
Human
NCBI GenBank
NM_018140
SwissProt
Q9P209

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
71.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MARAGPRLVL SEEAVRAKSG LGPHRDLAEL QSLSIPGTYQ EKITHLGHSL MSLTGLKSLD
61LSRNSLVSLE GIQYLTALES LNLYYNCISS LAEVFRLHAL TELVDVDFRL NPVVKVEPDY
121RLFVVHLLPK LQQLDDRPVR ASERKASRLH FASEDSLDSK ESVPASLKEG RPHHPRAKCT
181EALAKQSLVM DADDEAVLNL IAECEWDLGR PPGSTSFSQK GREADSRGSQ ESRHLLSPQL
241VQYQCGDSGK QGRETRRSSC RGCCLEKMPW SQLCGELPPL YGAEPEASRA PRPHTYFTPH
301PDSMDTEDSA SSQKLDLSGE MVPGPLPAPG KCRKRRMPVG RFQTFSDQEG LGCPERTHGS
361SVPKESLSRQ DSSESRNGRT LSQPEASETE EQRSRGVTDT REPSPGSHSA LPGKKTALQA
421ALLETLLDLV DRSWGGCRSL HSNEAFLAQA RHILSSVEEF TAAQDSSAMV GEDVGSLALE
481SKSLQSRLAE QQQQHAREMS EVTAELHHTH KELDDLRQHL DKSLEENSRL KSLLLSMKKE
541VKSADTAATL NLQIAGLQTS VKRLCGEIVE LKQHLEHYDK IQELTQMLQE SHSSLVSTNE
601HLLQELSQVR AQHRAEVEQM HWSYQELKKT MALFPHSSAS HGGCQACTRT RPLEQKLISE
661EDLAANDILD YKDDDDKV

678 aa

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.